Theurkauf W E
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794.
Science. 1994 Sep 30;265(5181):2093-6. doi: 10.1126/science.8091233.
Embryonic axis specification in Drosophila melanogaster is achieved through the asymmetric subcellular localization of morphogenetic molecules within the oocyte. The cappuccino and spire loci are required for both posterior and dorsoventral patterning. Time-lapse confocal microscopic analyses of living egg chambers demonstrated that these mutations induce microtubule reorganization and the premature initiation of microtubule-dependent ooplasmic streaming. As a result, microtubule organization is altered and bulk ooplasm rapidly streams during the developmental stages in which morphogens are normally localized. These changes in oocyte cytoarchitecture and dynamics appear to disrupt axial patterning of the embryo.
黑腹果蝇胚胎轴的特化是通过形态发生分子在卵母细胞内的不对称亚细胞定位来实现的。卡布奇诺基因座和纺锤体基因座对于前后轴和背腹轴模式的形成都是必需的。对活卵室进行的延时共聚焦显微镜分析表明，这些突变会诱导微管重组以及微管依赖性卵质流动的过早启动。结果，在形态发生素正常定位的发育阶段，微管组织发生改变，大量卵质迅速流动。卵母细胞细胞结构和动力学的这些变化似乎会破坏胚胎的轴向模式形成。
