McCririe L, Bairden K, Britton C, Buitkamp J, McKeand J B, Stear M J
Department of Veterinary Clinical Studies, Glasgow University, Scotland, UK.
Parasite Immunol. 1997 May;19(5):235-42. doi: 10.1046/j.1365-3024.1997.d01-202.x.
The recognition of parasite molecules from third-stage and adult Ostertagia circumcincta by serum antibody was studied in a group of matched, mature Scottish Blackface sheep that had been naturally and then deliberately infected. A total of 20 molecules was recognized in somatic extracts from third-stage larvae and 31 molecules in somatic extracts from adult parasites. However, no sheep recognized all immunogenic molecules and no molecule was recognized by all sheep. There was no obvious relationship between recognition of any parasite antigen and polymorphism at class I loci or at the DRBI class II locus of the major histocompatibility complex in these outbred animals. Only 15 molecules from third-stage larvae were present at a frequency suitable for statistical analysis and recognition of three of these 15 molecules was associated with differences in worm burdens. Recognition of two of five molecules from adult parasites was associated with differences in worm length. These results indicate that variation in the recognition of specific, identifiable parasite molecules may be partly responsible for variation among sheep in resistance to O. circumcincta.
在一组匹配的、成熟的苏格兰黑脸羊中，研究了血清抗体对三期和成虫阶段的环形奥斯特他线虫寄生虫分子的识别情况，这些羊先是自然感染，然后又特意进行了感染。在三期幼虫的体细胞提取物中总共识别出20种分子，在成虫寄生虫的体细胞提取物中识别出31种分子。然而，没有一只羊能识别所有的免疫原性分子，也没有一种分子能被所有的羊识别。在这些远交动物中，任何寄生虫抗原的识别与主要组织相容性复合体的I类基因座或DRBI II类基因座的多态性之间没有明显关系。三期幼虫中只有15种分子出现的频率适合进行统计分析，这15种分子中的3种分子的识别与虫负荷差异有关。成虫寄生虫的5种分子中的2种分子的识别与虫体长度差异有关。这些结果表明，对特定的、可识别的寄生虫分子的识别差异可能部分导致了绵羊对环形奥斯特他线虫抵抗力的差异。
