Brambilla G, Bocca A, Delise M, Guandalini E
Istituto Superiore di Sanità, Rome, Italy.
Vet Res Commun. 1994;18(1):37-42. doi: 10.1007/BF01839259.
There have been many studies on the efficacy of beta 2-adrenergic drugs as feed additives but no data are available at present on the use of clenbuterol in fish production. To evaluate the residues of clenbuterol in tissues of fish, 50 trout (Oncorhynchus mykiss) were fed for 21 days on a fish feed containing 5 ppm of the drug. The livers, muscles and skins of sample groups of fish were analysed by HPLC with visible spectrophotometric detection on days 15 and 21 of treatment and at intervals during a 30-day withdrawal time. Clenbuterol reached its highest levels in the liver (mean 440 ppb; SD = +/- 159; n = 5) on day 15 of treatment, with a slow depletion curve; 24 +/- 3 ppb was still present at the end of the withdrawal period. At this time, residues were still present in the edible tissues, i.e. muscle (5 +/- 1 ppb) and skin (7 +/- 3 ppb). Side-effects were noted during the first week of treatment.
关于β2 - 肾上腺素能药物作为饲料添加剂的功效已有许多研究，但目前尚无关于在鱼类生产中使用克伦特罗的数据。为了评估鱼类组织中克伦特罗的残留量，将50条虹鳟鱼（Oncorhynchus mykiss）投喂含5 ppm该药物的鱼饲料21天。在处理的第15天和第21天以及30天停药期内的不同时间间隔，对样本组鱼的肝脏、肌肉和皮肤进行高效液相色谱法结合可见分光光度检测分析。在处理的第15天，克伦特罗在肝脏中的含量达到最高水平（平均440 ppb；标准差=±159；n = 5），其消耗曲线较为缓慢；在停药期结束时仍有24±3 ppb存在。此时，可食用组织即肌肉（5±1 ppb）和皮肤（7±3 ppb）中仍有残留。在处理的第一周观察到了副作用。
