Renfrey S, Watts A
Department of Biochemistry, University of Oxford, UK.
Vaccine. 1994 Jun;12(8):747-52. doi: 10.1016/0264-410x(94)90227-5.
Four vaccines are available in the United Kingdom against influenza virus. All are subunit vaccines, defined as either split-virion or purified surface antigen vaccine; there are two of each distinct type available. Both vaccine types are less reactogenic than whole inactivated virus, with antigenicity induced by viral surface glycoproteins. Here, each of the four vaccines has been characterized by electron microscopy and SDS-PAGE analysis, giving a unique vaccine profile. Three vaccines contain internal viral nucleoprotein which, in the presence of residual haemagglutinin, may induce an influenza A virus cross-reactive cytotoxic T-cell response and thus be of value to vaccine efficacy. Residual lipid was present in three vaccines and recent evidence suggests that pyrogenicity is correlated with the presence of viral lipid with clusters of surface glycoproteins. By a combination of electron microscopic evidence and biochemical characterization, it has been possible to resolve compositional differences, not only between vaccine type, but also between each individual currently available vaccine. Hence, there is the possibility that the morphological differences characterized here may be contributory to potential reactogenic effects subsequent to vaccination.
在英国有四种针对流感病毒的疫苗。所有这些都是亚单位疫苗，定义为裂解病毒疫苗或纯化表面抗原疫苗；每种不同类型各有两种。这两种疫苗类型的反应原性均低于全灭活病毒，其抗原性由病毒表面糖蛋白诱导产生。在此，通过电子显微镜和SDS - PAGE分析对这四种疫苗中的每一种进行了表征，给出了独特的疫苗图谱。三种疫苗含有病毒内部核蛋白，在存在残余血凝素的情况下，可能诱导甲型流感病毒交叉反应性细胞毒性T细胞反应，因此对疫苗效力有价值。三种疫苗中存在残余脂质，最近的证据表明，热原性与带有表面糖蛋白簇的病毒脂质的存在相关。通过电子显微镜证据和生化表征相结合，不仅可以分辨不同疫苗类型之间的成分差异，还能分辨每种现有疫苗之间的差异。因此，这里所表征的形态差异有可能导致接种疫苗后潜在的反应原性效应。
