Hasui M, Hirabayashi Y, Kobayashi Y
Department of Pediatrics, Kansai Medical University, Osaka, Japan.
J Immunol Methods. 1989 Feb 8;117(1):53-8. doi: 10.1016/0022-1759(89)90118-x.
Bacterial ingestion and hydrogen peroxide production by polymorphonuclear leukocytes (PMN) were simultaneously measured using flow cytometry and 2',7'-dichlorofluorescein diacetate and fluorescent Staphylococcus aureus. Only 100 microliters of a whole blood specimen were required for these determinations, and the results were found to be independent of the absolute numbers of PMN, making the purification and adjustment of PMN numbers unnecessary. A positive correlation between phagocytosis and hydrogen peroxide production by individual PMN was demonstrated in healthy adults.
使用流式细胞术、2',7'-二氯荧光素二乙酸酯和荧光标记的金黄色葡萄球菌同时测定多形核白细胞（PMN）的细菌摄取和过氧化氢生成。这些测定仅需100微升全血标本，且结果发现与PMN的绝对数量无关，无需对PMN数量进行纯化和调整。在健康成年人中，单个PMN的吞噬作用与过氧化氢生成之间呈正相关。
