Czuppon A B, Kaplan V, Speich R, Baur X
Department of Allergology, Ruhr-University of Bochum, Germany.
Allergy. 1994 May;49(5):337-41. doi: 10.1111/j.1398-9995.1994.tb02278.x.
A 17-year-old man was occupationally exposed to pyromellitic acid dianhydride dust during the production of epoxy resin in a chemical factory. He was clinically diagnosed as having acute hemorrhagic alveolitis associated with anemia. The serologic analysis revealed a high concentration of IgG antibodies against pyromellitic acid dianhydride-treated human serum albumin (PMDA-HSA). Immunoblotting with PMDA-treated human serum as antigen and the patient's serum as the first antibody showed that additional PMDA-modified serum proteins other than HSA were recognized by the patient's IgG antibodies in the higher mol. mass range (> 67 kDa). No specific IgG could be detected against other anhydride conjugates (maleic acid, MA; phthalic acid, PA) with the exception of a reaction with the trimellitic acid anhydride-conjugated HSA (TMA-HSA). No specific IgE antibodies could be detected against any of the above mentioned antigens, but immunoblotting of the patient's serum indicated IgG4-type autoantibodies against in vitro PMDA-treated Ig molecules of normal serum proteins.
一名17岁男性在化工厂生产环氧树脂过程中职业性接触均苯四甲酸二酐粉尘。他临床诊断为急性出血性肺泡炎伴贫血。血清学分析显示，针对均苯四甲酸二酐处理的人血清白蛋白（PMDA - HSA）的IgG抗体浓度很高。以PMDA处理的人血清为抗原、患者血清为一抗进行免疫印迹分析表明，患者的IgG抗体在较高分子量范围（> 67 kDa）内识别出除HSA之外的其他PMDA修饰的血清蛋白。除了与偏苯三酸酐偶联的HSA（TMA - HSA）有反应外，未检测到针对其他酸酐偶联物（马来酸，MA；邻苯二甲酸，PA）的特异性IgG。未检测到针对上述任何抗原的特异性IgE抗体，但患者血清的免疫印迹显示存在针对体外PMDA处理的正常血清蛋白Ig分子的IgG4型自身抗体。
