Masuda H, Tanaka T, Takahama U
Department of Surgery, Kyushu Dental College, Japan.
Biochem Biophys Res Commun. 1994 Sep 15;203(2):1175-80. doi: 10.1006/bbrc.1994.2306.
Cisplatin [cis-Diamminedichloroplatinum (II), DDP] generated superoxide anion (O2-), as measured by chemiluminescence from a Cypridina luciferin analog (CLA), by interaction with calf thymus DNA (ct-DNA) in phosphate-buffered saline (PBS) at pH 7.6. The intensity of chemiluminescence of CLA (CCLA) increased with increased concentration of DDP, ct-DNA or CLA in the limited range of concentrations of these agents tested. DDP-induced CCLA was completely inhibited by the addition of an O2- scavenger, superoxide dismutase (SOD), or an antioxidant, ascorbic acid. Heat-inactivated SOD did not affect CCLA. The hydroxyl radical (OH.) scavengers, mannitol, ethanol, and formic acid, also caused a partial suppression of CCLA, suggesting that DDP produced OH. as well as O2- in the cell-free system used.
顺铂[顺-二氨二氯铂(II)，DDP]在pH 7.6的磷酸盐缓冲盐水(PBS)中与小牛胸腺DNA(ct-DNA)相互作用时，通过一种海萤荧光素类似物(CLA)的化学发光法检测，会产生超氧阴离子(O2-)。在测试的这些试剂的有限浓度范围内，CLA的化学发光强度(CCLA)随DDP、ct-DNA或CLA浓度的增加而增加。添加超氧阴离子清除剂超氧化物歧化酶(SOD)或抗氧化剂抗坏血酸可完全抑制DDP诱导的CCLA。热灭活的SOD不影响CCLA。羟基自由基(OH.)清除剂甘露醇、乙醇和甲酸也会部分抑制CCLA，这表明在所用的无细胞系统中，DDP会产生OH.以及O2-。
