Igel H J, Freeman A E, Spiewak J E, Kleinfeld K L
In Vitro. 1975 May-Jun;11(3):117-29. doi: 10.1007/BF02615420.
Seventy-five diploid human cell s-rains were subjected to a number of chemical carcinogens, including urethane and polycyclic hydrocarbons. In most cases, no visible morphological alterations were induced by any treatment. Development of morphologically altered foci was noticed in urethane-treated cultures derived from a patient with von Recklinghausen's disease. This disease is transmitted by an autosomal dominant gene, and has a high rate of spontaneous transformation of neurofibromas to neurofibrosarcomas. Attempts to isolate continuous cell lines from altered foci were successful in only two of several attempts. These continuous cell lines demonstrate altered morphology, loss of contact inhibition, accelerated growth rate, and have attained over 240 generations in a period of 140 weeks. Untreated control cultures became terminal by the 20th generation. Giemsa banding procedures showed that the chromosomal complement consisted of heteroploid human chromosomes. A second diploid cell strain derived from the above patient's sibling, also suffering from von Recklinghausen's disease, likewise was morphologically altered by urethane. Chemical transformation of human cells is difficult to induce; however, selection of genetically predisposed cells and prolonged, intermittent, and repeated chemical treatment may be important factors in achieving transformation.
75个二倍体人类细胞株接受了多种化学致癌物的处理，包括氨基甲酸乙酯和多环烃。在大多数情况下，任何处理都未诱导出可见的形态学改变。在源自一名患有冯·雷克林豪森病患者的经氨基甲酸乙酯处理的培养物中，注意到了形态学改变灶的形成。这种疾病由常染色体显性基因传递，并且神经纤维瘤自发转化为神经纤维肉瘤的发生率很高。从改变灶中分离连续细胞系的尝试在几次尝试中仅成功了两次。这些连续细胞系表现出形态改变、接触抑制丧失、生长速率加快，并且在140周的时间内传代超过240次。未经处理的对照培养物在第20代时进入终末状态。吉姆萨显带程序显示染色体组由异倍体人类染色体组成。源自上述同样患有冯·雷克林豪森病的患者同胞的第二个二倍体细胞株，同样也被氨基甲酸乙酯诱导发生了形态改变。人类细胞的化学转化很难诱导；然而，选择具有遗传易感性的细胞以及延长、间歇和重复的化学处理可能是实现转化的重要因素。
