Nakashima Y, Yuasa S, Hukamizu Y, Okuyama H, Ohhara T, Kameyama T, Nabeshima T
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
J Lipid Res. 1993 Feb;34(2):239-47.
Semi-purified diets supplemented with either a high linoleate (n-6) (safflower) oil or a high alpha-linolenate (n-3) (perilla) oil were fed to mouse mothers and their offspring through 6 weeks of age. The proportions of n-3 and n-6 highly unsaturated fatty acids in brain phospholipids reflected the n-3/n-6 balance of the diets while no difference was found in phospholipid compositions or cholesterol/phospholipid ratios. In the elevated plus maze task, the total number of entries into the open- and enclosed-arms was smaller and the time spent in the dark enclosed arms tended to be longer in the perilla group than the safflower group. The time required to reach a safe platform in Morris's water maze test was less in the perilla group, but no significant difference was observed in the entries into the arms darkened with a movable cover in Y-maze dark-preference task. The safflower group was more sensitive to pentobarbital; the anesthesia onset time was less and the anesthetic time was longer than in the perilla group. Increased locomotion induced by scopolamine injection was less in the safflower group as compared with the perilla group. These results indicate that in mice the dietary alpha-linolenate/linoleate balance affects the n-3/n-6 ratio of brain phospholipid acyl chains and that this is accompanied by general behavioral changes as well as changes in sensitivities to drugs known to affect behavior.
给小鼠母亲及其后代喂食补充了高亚油酸（n-6）（红花）油或高α-亚麻酸（n-3）（紫苏）油的半纯化日粮，直至6周龄。脑磷脂中n-3和n-6高度不饱和脂肪酸的比例反映了日粮的n-3/n-6平衡，而磷脂组成或胆固醇/磷脂比率没有差异。在高架十字迷宫试验中，与红花组相比，紫苏组进入开放臂和封闭臂的总次数较少，且在黑暗封闭臂中停留的时间往往更长。在莫里斯水迷宫试验中，紫苏组到达安全平台所需的时间较少，但在Y迷宫暗偏好试验中，进入用可移动盖子变暗的臂的次数没有显著差异。红花组对戊巴比妥更敏感；与紫苏组相比，麻醉起效时间更短，麻醉时间更长。与紫苏组相比，红花组注射东莨菪碱引起的运动增加较少。这些结果表明，在小鼠中，日粮α-亚麻酸/亚油酸平衡影响脑磷脂酰基链的n-3/n-6比率，并且这伴随着一般行为变化以及对已知影响行为的药物敏感性的变化。
