Arko R J, Odugbemi T
J Clin Microbiol. 1984 Jul;20(1):1-4. doi: 10.1128/jcm.20.1.1-4.1984.
Two inexpensive screening tests were evaluated singly and in tandem for distinguishing Neisseria gonorrhoeae from other oxidase-positive microorganisms growing on selective gonococcal media. In tests of 728 cultures, including 460 N. gonorrhoeae, 4 Neisseria lactamica, 257 Neisseria meningitidis, and 7 Branhamella catarrhalis, both Superoxol (30% H2O2; J. T. Baker Chemical Co., Phillipsburg, N.J.) and amylase inhibition tests were 100% sensitive (positive) for 20-h cultures of N. gonorrhoeae. Singly, the Superoxol test was 92.7% specific for N. gonorrhoeae, compared with a specificity of 82.3% for the amylase inhibition test. By using tandem screening tests to distinguish gonococci, we achieved an overall specificity of 98.6%. Group A meningococci were the primary source of error in the Superoxol test, with 97% (37 of 38) strains producing gonococcal like reactions for catalase. From 5 to 20% of N. meningitidis serogroups X, Y, Z, and Z' and nontypable strains, as well as about 50% of B. catarrhalis and N. lactamica strains, were also strong catalase producers.
对两种低成本筛查试验进行了单独及串联评估，以区分淋病奈瑟菌与在选择性淋球菌培养基上生长的其他氧化酶阳性微生物。在对728份培养物的检测中，包括460份淋病奈瑟菌、4份乳酸奈瑟菌、257份脑膜炎奈瑟菌和7份卡他布兰汉菌，对于淋病奈瑟菌的20小时培养物，超氧化物试验（30%过氧化氢；J.T.贝克化学公司，新泽西州菲利普斯堡）和淀粉酶抑制试验的敏感性均为100%（阳性）。单独来看，超氧化物试验对淋病奈瑟菌的特异性为92.7%，而淀粉酶抑制试验的特异性为82.3%。通过使用串联筛查试验来区分淋球菌，我们实现了98.6%的总体特异性。A群脑膜炎球菌是超氧化物试验中的主要误差来源，97%（38份中的37份）菌株的过氧化氢酶产生淋病奈瑟菌样反应。脑膜炎奈瑟菌血清群X、Y、Z和Z'以及不可分型菌株的5%至20%，以及约50%的卡他布兰汉菌和乳酸奈瑟菌菌株也是强过氧化氢酶产生菌。
