Bayliss C E, Waites W M
J Gen Microbiol. 1976 Oct;96(2):401-7. doi: 10.1099/00221287-96-2-401.
The effect of hydrogen peroxide on the germination, colony formation and structure of spores of Clostridium bifermentans was examined. Treatment with 0.35 M-hydrogen peroxide increased the germination rate at 25 degrees C but increasing the temperature or concentration of hydrogen peroxide decreased both the germination rate and colony formation. The presence of Cu2+ increased the lethal effect of hydrogen peroxide on colony formation as much as 3000-fold. Pre-incubation of spores with Cu2+ before treatment with hydrogen peroxide produced a similar increase, but this could be eliminated by washing the spores with dilute spores--apparently from the coat--and treatment with dithiothreitol, which also removes spore-coat protein, increased the lethal effect of hydrogen peroxide 500-fold, suggesting that spore-coat protein has a protective effect against hydrogen peroxide.
研究了过氧化氢对双发酵梭菌孢子萌发、菌落形成及结构的影响。用0.35M过氧化氢处理可提高25℃时的萌发率，但升高温度或增加过氧化氢浓度会降低萌发率和菌落形成。Cu2+的存在使过氧化氢对菌落形成的致死效应增加多达3000倍。在用过氧化氢处理之前，将孢子与Cu2+预孵育也会产生类似的增加，但这可以通过用稀释的孢子——显然来自孢子外壁——洗涤孢子来消除，用二硫苏糖醇处理（二硫苏糖醇也可去除孢子外壁蛋白）会使过氧化氢的致死效应增加500倍，这表明孢子外壁蛋白对过氧化氢具有保护作用。
