Ye D W, Zheng J F, Qian S X, Ma Y J
Department of Urology, Changhai Hospital, People's Republic of China.
Urol Res. 1993 Jan;21(1):39-43. doi: 10.1007/BF00295190.
Expression of the ras and the c-erbB-2 oncogene products was investigated in 56 cases of human bladder transitional cell carcinoma and 6 samples of human normal bladder tissue using an immunohistochemical method. Thirty of the 56 cases of bladder tumor were found to be immunohistologically positive with the monoclonal anti-ras p21 antibody, while 19 of 56 cases were positive with the polyclonal anti-c-erbB-2 oncoprotein antibody. All 6 controls were negative with both antibodies. The ras p21 positive staining was found more frequently in the well or moderately differentiated, superficial and non-recurrent tumors than in the poorly differentiated (p < 0.01), muscle invasive (p < 0.05) and recurrent tumors (p < 0.01), while the c-erbB-2 gene product was more commonly detected in high-grade (p < 0.01), invasive (p < 0.01) and recurrent tumors (p < 0.05). Thus, the expression of either ras or c-erbB-2 was closely associated with the histological grade, clinical stage and recurrence of bladder transitional cell carcinomas.
采用免疫组织化学方法，对56例人膀胱移行细胞癌和6例人正常膀胱组织样本中的ras和c-erbB-2癌基因产物表达情况进行了研究。56例膀胱肿瘤中，30例经免疫组织化学检测，单克隆抗ras p21抗体呈阳性，而56例中有19例多克隆抗c-erbB-2癌蛋白抗体呈阳性。所有6例对照两种抗体均为阴性。ras p21阳性染色在高分化或中分化、表浅及非复发性肿瘤中比低分化肿瘤（p<0.01）、肌层浸润性肿瘤（p<0.05）及复发性肿瘤（p<0.01）中更常见，而c-erbB-2基因产物在高级别肿瘤（p<0.01）、浸润性肿瘤（p<0.01）及复发性肿瘤（p<0.05）中更常检测到。因此，ras或c-erbB-2的表达与膀胱移行细胞癌的组织学分级、临床分期及复发密切相关。
