Gruner E, Kropec A, Huebner J, Altwegg M, Daschner F
Institute of Medical Microbiology, University of Zurich, Switzerland.
J Infect Dis. 1993 May;167(5):1216-20. doi: 10.1093/infdis/167.5.1216.
To elucidate the sources of Pseudomonas aeruginosa on a surgical intensive care unit, rDNA restriction fragment length polymorphism analysis (ribotyping) was applied to analyze strains isolated during a 4-month prospective study. Samples included 1635 from 153 patients, 2463 from 97 staff members, and 581 from the environment. Only 18 patients were colonized. Isolation from their animate and inanimate environment was very low, with 3 and 2 samples, respectively, being positive. Samples from tap water were negative. Ribotyping could easily distinguish 16 different digest patterns with identical follow-up isolates of the same patient. Horizontal transmission occurred only twice. The discriminatory power of ribosomal DNA in differentiating strains was dependent on the restriction enzymes used; among eight different enzymes, PvuII was the most sensitive, producing 15 different patterns. Ribotyping showed high sensitivity in typing P. aeruginosa isolates and confirmed that colonization occurs from endogenous rather than from exogenous sources.
为阐明外科重症监护病房铜绿假单胞菌的来源，应用核糖体DNA限制性片段长度多态性分析（核糖分型）对一项为期4个月的前瞻性研究期间分离出的菌株进行分析。样本包括来自153例患者的1635份样本、来自97名工作人员的2463份样本以及来自环境的581份样本。仅18例患者发生定植。从其有生命和无生命环境中的分离率非常低，分别只有3份和2份样本呈阳性。自来水样本为阴性。核糖分型能够轻松区分16种不同的酶切模式，同一患者的后续分离株具有相同模式。水平传播仅发生过两次。核糖体DNA区分菌株的鉴别能力取决于所使用的限制性酶；在8种不同的酶中，PvuII最为敏感，可产生15种不同模式。核糖分型在对铜绿假单胞菌分离株进行分型时显示出高敏感性，并证实定植源于内源性而非外源性来源。
