间叶组织肿瘤中生长因子受体及相关癌基因的测定
Growth factor receptor and related oncogene determination in mesenchymal tumors.
作者信息
Duda R B, Cundiff D, August C Z, Wagman L D, Bauer K D
机构信息
Division of Surgical Oncology, Harvard Medical School, Beth Israel Hospital, Boston, Massachusetts 02215.
出版信息
Cancer. 1993 Jun 1;71(11):3526-30. doi: 10.1002/1097-0142(19930601)71:11<3526::aid-cncr2820711111>3.0.co;2-q.
BACKGROUND
Sarcoma are rare malignant neoplasms that originate from a mesenchymal cell line. The epidermal growth factor receptor (EGF-R) has been identified in these malignant neoplasms by immunohistochemical techniques.
METHODS
This investigation has evaluated the gene amplification and expression of EGF-R and the homologous oncogene c-erbB-2 in soft tissue and osseous sarcomas by Southern and northern blot analysis.
RESULTS
Amplification of EGF-R and c-erbB-2 was identified in 2 of 117 (1.7%) and 6 of 105 (5.7%) of the sarcomas, respectively. Increased expression of EGF-R and c-erbB-2 was identified in 21 of 43 (49%) and 35 of 94 (37%) sarcomas, respectively.
CONCLUSIONS
The expression of these two genes in sarcomas appears to occur independently and not be associated with tumor histologic characteristics, grade, size, DNA content, or proliferative activity.
背景
肉瘤是起源于间充质细胞系的罕见恶性肿瘤。通过免疫组化技术已在这些恶性肿瘤中鉴定出表皮生长因子受体（EGF-R）。
方法
本研究通过Southern和Northern印迹分析评估了软组织和骨肉瘤中EGF-R及同源癌基因c-erbB-2的基因扩增和表达情况。
结果
在117例肉瘤中的2例（1.7%）和105例肉瘤中的6例（5.7%）分别检测到EGF-R和c-erbB-2的扩增。在43例肉瘤中的21例（49%）和94例肉瘤中的35例（37%）分别检测到EGF-R和c-erbB-2的表达增加。
结论
这两个基因在肉瘤中的表达似乎是独立发生的，且与肿瘤的组织学特征、分级、大小、DNA含量或增殖活性无关。
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