Morita M
Department of Pediatrics, Faculty of Medicine, Kyoto University.
Nihon Rinsho. 1993 Mar;51(3):712-7.
Recently, a human eosinophilic leukemia cell line, EoL was established from an eosinophilic leukemia patient. EoL-1 cells have the cytohistologic features of myeloblasts under normal culture conditions, and they can be induced to differentiate into eosinophilic granule-containing cells but not into other lineage cells under several culture conditions and are therefore considered to be committed precursors of eosinophils. Furthermore, EoL-1 cells can also be induced to differentiate functionally to show PAF-induced Ca2+ influx and actin polymerization. On the other hand, EoL-3 cells show constitutive expression of Fc epsilon RII, Fc gamma RII, LFA-1 and ICAM-1 on their cell surface. The EoL cells may provide new information on some aspects of the signal transduction mechanisms involved in the proliferation, differentiation and activation of eosinophils.
最近，一种人类嗜酸性粒细胞白血病细胞系EoL从一名嗜酸性粒细胞白血病患者体内建立。在正常培养条件下，EoL-1细胞具有成髓细胞的细胞组织学特征，在几种培养条件下，它们可被诱导分化为含嗜酸性粒细胞颗粒的细胞，但不能分化为其他谱系细胞，因此被认为是嗜酸性粒细胞的定向前体。此外，EoL-1细胞还可被诱导发生功能分化，表现出PAF诱导的Ca2+内流和肌动蛋白聚合。另一方面，EoL-3细胞在其细胞表面组成性表达FcεRII、FcγRII、LFA-1和ICAM-1。EoL细胞可能为嗜酸性粒细胞增殖、分化和激活所涉及的信号转导机制的某些方面提供新信息。
