Thierry D, Matsiota-Bernard P, Pitsouni E, Costopoulos C, Guesdon J L
Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.
FEMS Immunol Med Microbiol. 1993 Apr;6(4):287-97. doi: 10.1111/j.1574-695X.1993.tb00341.x.
IS6100 is an insertion sequence of the IS3 family and it is present in multiple copies in the chromosome of Mycobacterium tuberculosis. Four to 15 copies are present in various strains of M. tuberculosis. In this study, the value of IS6110 as an epidemiological marker of tuberculosis was examined. Unrelated clinical strains from Greek patients presented, in restriction fragment length polymorphism analysis, a high degree of polymorphism, whereas patterns of related clinical strains from familial outbreaks were identical. Since RFLP analysis with acetylaminofluorene labeled IS6110 as the probe gave satisfactory results, it is suggested that this non-radioactive probe can be used in hospitals and health centres for the epidemiological survey of M. tuberculosis infections.
IS6100是IS3家族的一种插入序列，在结核分枝杆菌染色体中以多拷贝形式存在。在不同的结核分枝杆菌菌株中存在4到15个拷贝。在本研究中，检测了IS6110作为结核病流行病学标志物的价值。在限制性片段长度多态性分析中，来自希腊患者的不相关临床菌株呈现出高度多态性，而来自家族性暴发的相关临床菌株的模式则是相同的。由于以乙酰氨基芴标记的IS6110作为探针进行的RFLP分析给出了满意的结果，因此建议这种非放射性探针可用于医院和卫生中心对结核分枝杆菌感染进行流行病学调查。
