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使用IS6110作为结核流行病学标志物的限制性片段长度多态性分析。

Restriction fragment length polymorphism analysis using IS6110 as an epidemiological marker in tuberculosis.

作者信息

Otal I, Martín C, Vincent-Lévy-Frebault V, Thierry D, Gicquel B

机构信息

Unité Génie Microbiologique, Centre National de la Recherche Scientifique, Paris, France.

出版信息

J Clin Microbiol. 1991 Jun;29(6):1252-4. doi: 10.1128/jcm.29.6.1252-1254.1991.

DOI:10.1128/jcm.29.6.1252-1254.1991
PMID:1677943
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269979/
Abstract

The mycobacterial insertion sequence IS6110 has been shown to be present in multiple copies in the chromosome of Mycobacterium tuberculosis. IS6110 restriction fragment length polymorphism analysis of strains isolated from patients who developed tuberculosis showed identical patterns over a 2- to 3-year period. In contrast, a high degree of polymorphism was observed between strains of the M. tuberculosis complex isolated from different patients. This study demonstrates that the presence of IS6110 does not induce in vivo major genomic rearrangements over a 2- to 3-year period and confirms its use as a valuable epidemiological marker in tuberculosis.

摘要

分枝杆菌插入序列IS6110已被证明在结核分枝杆菌染色体中以多拷贝形式存在。对从患结核病患者中分离出的菌株进行IS6110限制性片段长度多态性分析，结果显示在2至3年期间模式相同。相比之下，从不同患者中分离出的结核分枝杆菌复合群菌株之间观察到高度多态性。本研究表明，在2至3年期间，IS6110的存在不会在体内诱导主要的基因组重排，并证实了其作为结核病中有价值的流行病学标志物的用途。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/576a/269979/d2b0eda237e4/jcm00042-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/576a/269979/d2b0eda237e4/jcm00042-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/576a/269979/d2b0eda237e4/jcm00042-0179-a.jpg

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