Hjelle B, Jenison S, Torrez-Martinez N, Herring B, Quan S, Polito A, Pichuantes S, Yamada T, Morris C, Elgh F, Lee H W, Artsob H, Dinello R
Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131-5301, USA.
J Clin Microbiol. 1997 Mar;35(3):600-8. doi: 10.1128/jcm.35.3.600-608.1997.
To develop a rapid antibody test for Sin Nombre hantavirus (SNV) infection for diagnosis of hantavirus pulmonary syndrome (HPS) in field settings where advanced instrumentation is not available, a strip immunoblot assay bearing four immobilized antigens for SNV and a recombinant nucleocapsid protein antigen of Seoul hantavirus (SEOV) was prepared. The SNV antigens included a full-length recombinant-expressed nucleocapsid (N) protein (rN), a recombinant-expressed G1 protein (residues 35 to 117), and synthetic peptides derived from N (residues 17 to 59) and G1 (residues 55 to 88). On the basis of the observed reactivities of hantavirus-infected patient and control sera, we determined that a positive assay requires reactivity with SNV or SEOV rN antigen and at least one other antigen. Isolated reactivity to either viral rN antigen is indeterminate, and any pattern of reactivity that does not include reactivity to an rN antigen is considered indeterminate but is unlikely to represent hantavirus infection. Fifty-eight of 59 samples from patients with acute SNV-associated HPS were positive according to these criteria, and one was initially indeterminate. Four of four samples from patients with HPS due to other hantaviruses were positive, as were most samples from patients with SEOV and Puumala virus infections. Of 192 control serum samples, 2 (1%) were positive and 2 were indeterminate. Acute SNV infection was distinguishable from remote SNV infection or infection with hantaviruses other than SNV by the presence of G1 peptide antigen reactivities in the former. The strip immunoblot assay shows promise for the detection of SNV antibodies early in the course of HPS.
为开发一种用于诊断汉坦病毒肺综合征（HPS）的快速抗体检测方法，以用于在没有先进仪器的现场环境中检测辛诺柏病毒（SNV）感染，制备了一种条带免疫印迹检测法，该检测法带有四种用于SNV的固定抗原以及汉城病毒（SEOV）的重组核衣壳蛋白抗原。SNV抗原包括全长重组表达的核衣壳（N）蛋白（rN）、重组表达的G1蛋白（第35至117位氨基酸残基）以及源自N（第17至59位氨基酸残基）和G1（第55至88位氨基酸残基）的合成肽。根据观察到的感染汉坦病毒患者血清和对照血清的反应性，我们确定阳性检测结果需要与SNV或SEOV rN抗原以及至少一种其他抗原发生反应。仅与病毒rN抗原发生反应的结果不确定，任何不包括与rN抗原发生反应的反应模式都被视为不确定，但不太可能代表汉坦病毒感染。根据这些标准，59例急性SNV相关HPS患者的样本中有58例呈阳性，1例最初结果不确定。4例因其他汉坦病毒导致HPS的患者样本均呈阳性，SEOV和普马拉病毒感染患者的大多数样本也呈阳性。在192份对照血清样本中，2份（1%）呈阳性，2份结果不确定。急性SNV感染可通过前者中存在G1肽抗原反应性与既往SNV感染或SNV以外的其他汉坦病毒感染相区分。条带免疫印迹检测法在HPS病程早期检测SNV抗体方面显示出前景。
