Meuret G, Batara E, Fürste H O
Acta Haematol. 1975;54(5):261-70. doi: 10.1159/000208084.
Monocytopoiesis was analyzed in the bone marrow of healthy individuals. Promonocytes were identified by simultaneous determination of sodium-fluoride-sensitive and resistent naphthol-AS-D-acetate esterases. DNA synthesis activity of enzyme-positive promocytes was determined by 3H-thymidine (3H-TDR) incorporation in vitro. DNA synthesis time of these cells was measured by a double labelling technique (3H-TDR in vivo, 14C-TDR in vitro) as well as by serial injections of 3H-TDR. The relative number of promonocytes in the myelogram averaged 2.9% corresponding to a medullar promonocyte pool of about 600 X 10(6) cells per kilogram body weight. The promonocytes were classified into 4 groups on the basis of nucleus morphology: type I promonocytes with small lymphocyte-like nuclei (mean frequency of occurence, F = 5%; mean 3H-TDR labelling index, LI = 7.1%; type II promonocytes with large round or oval nuclei (F = 31%; LI = 9.7%); type III promonocytes with large, slightly folded nuclei (F = 51%; LI = 10.1%); type IV promonocytes with large, distinctly folded nuclei (F = 13%; LI = 24.9%). LI of pooled promonocytes was 12.0%. Mean DNA synthesis times of the different types of promonocytes was similar and approximated 10 h (range 6.6-13.3 h). This was true under normal conditions as well as in septicaemia.
对健康个体的骨髓中的单核细胞生成进行了分析。通过同时测定对氟化钠敏感和抗性的萘酚-AS-D-醋酸酯酶来鉴定原单核细胞。通过体外掺入3H-胸腺嘧啶核苷（3H-TDR）来测定酶阳性原单核细胞的DNA合成活性。这些细胞的DNA合成时间通过双标记技术（体内3H-TDR，体外14C-TDR）以及连续注射3H-TDR来测量。骨髓象中原单核细胞的相对数量平均为2.9%，相当于每千克体重约600×10⁶个细胞的骨髓原单核细胞池。根据细胞核形态，将原单核细胞分为4组：I型原单核细胞，具有小淋巴细胞样核（平均出现频率，F = 5%；平均3H-TDR标记指数，LI = 7.1%）；II型原单核细胞，具有大的圆形或椭圆形核（F = 31%；LI = 9.7%）；III型原单核细胞，具有大的、轻度折叠的核（F = 51%；LI = 10.1%）；IV型原单核细胞，具有大的、明显折叠的核（F = 13%；LI = 24.9%）。汇集的原单核细胞的LI为12.0%。不同类型原单核细胞的平均DNA合成时间相似，约为10小时（范围6.6 - 13.3小时）。在正常条件下以及败血症中都是如此。
