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Deficiens，一种参与控制金鱼草花形态发生的同源异型基因：该蛋白质与转录因子具有同源性。

Deficiens, a homeotic gene involved in the control of flower morphogenesis in Antirrhinum majus: the protein shows homology to transcription factors.

作者信息

Sommer H, Beltrán J P, Huijser P, Pape H, Lönnig W E, Saedler H, Schwarz-Sommer Z

机构信息

Max-Planck-Institut für Züchtungsforschung, Köln, FRG.

出版信息

EMBO J. 1990 Mar;9(3):605-13. doi: 10.1002/j.1460-2075.1990.tb08152.x.

DOI:10.1002/j.1460-2075.1990.tb08152.x
PMID:1968830
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC551713/
Abstract

Deficiens (defA+) is a homeotic gene involved in the genetic control of Antirrhinum majus flower development. Mutation of this gene (defA-1) causes homeotic transformation of petals into sepals and of stamina into carpels in flowers displaying the 'globifera' phenotype, as shown by cross sections and scanning electronmicroscopy of developing flowers. A cDNA derived from the wild type defA+ gene has been cloned by differential screening of a subtracted 'flower specific' cDNA library. The identity of this cDNA with the defA+ gene product has been confirmed by utilizing the somatic and germinal instability of defA-1 mutants. According to Northern blot analyses the defA+ gene is expressed in flowers but not in leaves, and its expression is nearly constant during all stages of flower development. The 1.1 kb long mRNA has a 681 bp long open reading frame that can code for a putative protein of 227 amino acids (mol. wt 26.2 kd). At its N-terminus the DEF A protein reveals homology to a conserved domain of the regulatory proteins SRF (activating c-fos) in mammals and GRM/PRTF (regulating mating type) in yeast. We discuss the structure and the possible function of the DEF A protein in the control of floral organogenesis.

摘要

Deficiens（defA+）是一个参与金鱼草花发育遗传控制的同源异型基因。该基因（defA-1）发生突变会导致呈现“globifera”表型的花朵中花瓣向萼片、雄蕊向心皮的同源异型转变，发育中的花朵的横截面和扫描电子显微镜观察结果表明了这一点。通过对消减的“花特异性”cDNA文库进行差异筛选，克隆出了源自野生型defA+基因的cDNA。利用defA-1突变体的体细胞和生殖细胞不稳定性，证实了该cDNA与defA+基因产物的一致性。根据Northern印迹分析，defA+基因在花中表达，但在叶中不表达，并且在花发育的所有阶段其表达几乎恒定。1.1 kb长的mRNA具有一个681 bp长的开放阅读框，可编码一个推定的由227个氨基酸组成的蛋白质（分子量26.2 kd）。DEF A蛋白在其N端显示出与哺乳动物中调节蛋白SRF（激活c-fos）和酵母中GRM/PRTF（调节交配型）的保守结构域具有同源性。我们讨论了DEF A蛋白在花器官发生控制中的结构和可能功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a368/551713/1ecb301e05a7/emboj00230-0021-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a368/551713/91ad02c0a444/emboj00230-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a368/551713/b12fa68a951a/emboj00230-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a368/551713/57fae2c00b95/emboj00230-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a368/551713/1ecb301e05a7/emboj00230-0021-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a368/551713/91ad02c0a444/emboj00230-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a368/551713/b12fa68a951a/emboj00230-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a368/551713/57fae2c00b95/emboj00230-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a368/551713/1ecb301e05a7/emboj00230-0021-b.jpg

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Deficiens, a homeotic gene involved in the control of flower morphogenesis in Antirrhinum majus: the protein shows homology to transcription factors.Deficiens，一种参与控制金鱼草花形态发生的同源异型基因：该蛋白质与转录因子具有同源性。
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