Parry T J, McElligott J G
Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140.
Physiol Behav. 1993 May;53(5):1011-5. doi: 10.1016/0031-9384(93)90283-l.
A method for restraining awake rats using head implant immobilization is described. In order to reduce stress to the individual animal, rats were restrained side by side in pairs during the adaptation and experimental periods. This technique was used in studies of central regulation of cardiovascular function. In particular, microdialysis probes were placed stereotaxically in deep nuclei of the cerebellum while mean arterial pressure and heart rate were monitored in the awake rat. After initial habituation, normal levels of heart rate and blood pressure obtained during restraint indicated that this is essentially a nonstressful procedure. This technique could also be used for a variety of other experimental conditions where head movement is not desirable, such as during oculomotor or vestibular experiments.
描述了一种使用头部植入固定法限制清醒大鼠活动的方法。为了减轻对单个动物的压力，在适应期和实验期将大鼠成对并排限制活动。该技术用于心血管功能的中枢调节研究。具体而言，将微透析探针立体定位放置在小脑深部核团，同时监测清醒大鼠的平均动脉压和心率。在最初的适应期过后，限制活动期间获得的正常心率和血压水平表明这基本上是一个无应激的过程。该技术还可用于各种其他不需要头部移动的实验条件，例如在动眼神经或前庭实验期间。
