一种新型立体定位仪，用于清醒状态下头固定大鼠的神经元记录。
A novel stereotaxic apparatus for neuronal recordings in awake head-restrained rats.
机构信息
Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23298-0599, USA.
出版信息
J Neurosci Methods. 2011 May 15;198(1):29-35. doi: 10.1016/j.jneumeth.2011.02.025. Epub 2011 Mar 15.
Abstract
A novel technique for neuronal recordings in awake head-restrained animals is presented. Our setup allows (1) daily repeat microelectrode studies in rats without use of anesthesia, (2) excellent stabilization of head using an eight point fixation, (3) painless head-restraint of the animal, (4) accurate stereotaxic localization during multiple sessions of recording, (5) to considerably reduced surgical time, (6) quick repositioning during chronic recording sessions and (7) high quality stabilized neuronal recordings during periods of rest and active movements.
摘要
本文介绍了一种在清醒束缚动物中进行神经元记录的新方法。我们的设置允许：（1）在不使用麻醉的情况下，每日重复对大鼠进行微电极研究；（2）通过八点固定实现头部的极佳稳定性；（3）对动物进行无痛束缚；（4）在多次记录过程中实现精确的立体定位；（5）大大减少手术时间；（6）在慢性记录过程中快速重新定位；（7）在休息和主动运动期间进行高质量的稳定神经元记录。
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