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[在DNA分析范围内测定限制性片段长度时的测量精度]

[Measuring precision in determining the length of restriction fragments within the scope of DNA analysis].

作者信息

Bässler G, Eberspächer B, Linder S, Pflug W

机构信息

Kriminaltechnischen Institut des Landeskriminalamtes, Baden-Württemberg, Stuttgart.

出版信息

Arch Kriminol. 1993 Mar-Apr;191(3-4):89-98.

PMID:8099786
Abstract

Because of the measurement error that is known from the length determination of restriction fragments during DNA analysis, it is necessary to determine the precision by which identical DNA can be evaluated. Besides the collection of frequency data from a certain population this is one of the basic prerequisites to calculate the likelihood ratio of an established polymorphism. By multiple measurement of restriction fragments from different persons indicated by the probes MS1, MS31, MS43A, g3 and YNH24 on different blots the within-laboratory variation was determined in the complete separation area from 1 to 19 kb. The values were compared to procedures used by other laboratories.

摘要

由于在DNA分析过程中,限制片段长度测定存在已知的测量误差,因此有必要确定评估相同DNA的精确程度。除了从特定人群中收集频率数据外,这也是计算既定多态性似然比的基本前提之一。通过对不同印迹上由探针MS1、MS31、MS43A、g3和YNH24指示的不同人员的限制片段进行多次测量,在1至19 kb的完全分离区域内确定了实验室内的变异情况。将这些值与其他实验室使用的程序进行了比较。

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