Weide R, van Wordragen M F, Lankhorst R K, Verkerk R, Hanhart C, Liharska T, Pap E, Stam P, Zabel P, Koornneef M
Department of Molecular Biology, Wageningen Agricultural University, The Netherlands.
Genetics. 1993 Dec;135(4):1175-86. doi: 10.1093/genetics/135.4.1175.
In the past, a classical map of the tomato genome has been established that is based on linkage data from intraspecific Lycopersicon esculentum crosses. In addition, a high density molecular linkage map has recently been constructed using a L. esculentum x L. pennellii cross. As the respective maps only partially match, they provide limited information about the relative positions of classical and molecular markers. In this paper we describe the construction of an integrated linkage map of tomato chromosome 6 that shows the position of cDNA-, genomic DNA- and RAPD markers relative to 10 classical markers. Integration was achieved by using a L. esculentum line containing an introgressed chromosome 6 from L. pennellii in crosses to a variety of L. esculentum marker lines. In addition, an improved version of the classical linkage map is presented that is based on a combined analysis of new linkage data for 16 morphological markers and literature data. Unlike the classical map currently in use, the revised map reveals clustering of markers into three major groups around the yv, m-2 and c loci, respectively. Although crossing-over rates are clearly different when comparing intraspecific L. esculentum crosses with L. esculentum x L. pennellii crosses, the clusters of morphological markers on the classical map coincide with clusters of genomic- and cDNA-markers on the molecular map constructed by Tanksley and coworkers.
过去，基于种内番茄（Lycopersicon esculentum）杂交的连锁数据构建了番茄基因组的经典图谱。此外，最近利用番茄（L. esculentum）与潘那利番茄（L. pennellii）的杂交构建了高密度分子连锁图谱。由于各自的图谱仅部分匹配，它们提供的关于经典标记和分子标记相对位置的信息有限。在本文中，我们描述了番茄6号染色体整合连锁图谱的构建，该图谱显示了cDNA、基因组DNA和RAPD标记相对于10个经典标记的位置。通过使用一个含有来自潘那利番茄渐渗6号染色体的番茄品系与多种番茄标记品系杂交来实现整合。此外，还展示了基于对16个形态学标记的新连锁数据和文献数据的综合分析得到的经典连锁图谱的改进版本。与目前使用的经典图谱不同，修订后的图谱揭示了标记分别在yv、m - 2和c位点周围聚集成三个主要组。尽管种内番茄杂交与番茄×潘那利番茄杂交的交换率明显不同，但经典图谱上的形态学标记簇与坦克斯利及其同事构建的分子图谱上的基因组和cDNA标记簇是一致的。
