Crawford R L
Appl Microbiol. 1975 Sep;30(3):439-44. doi: 10.1128/am.30.3.439-444.1975.
The pathway whereby certain bacterial strains of the genus Bacillus degrade m-hydroxybenzoate is delineated. Of 12 strains examined, nine were tentatively classified as representatives of the species Bacillus brevis, two of Bacillus sphaericus and one of Bacillus megaterium. All strains degraded m-hydroxybenzoate via the same pathway. m-Hydroxybenzoate was hydroxylated to 2,5-dihydroxybenzoate (gentisate), which was oxidized by a gentisate 1,2-deoxygenase yielding maleylpyruvate. Maleylpyruvate was hydrolyzed without prior cis, cis to cis, trans isomerization yielding pyruvate and maleic acid. Numerous soils were examined by plate-count procedures and found to contain 10(4) to 10(6) aerobic sporeformers able to grow on m-hydroxybenzoate per g of dry soil.
已阐明芽孢杆菌属某些菌株降解间羟基苯甲酸的途径。在所检测的12个菌株中,9个被初步归类为短短芽孢杆菌的代表菌株,2个为球形芽孢杆菌,1个为巨大芽孢杆菌。所有菌株均通过相同途径降解间羟基苯甲酸。间羟基苯甲酸被羟基化为2,5 - 二羟基苯甲酸(龙胆酸),后者被龙胆酸1,2 - 脱氧酶氧化生成马来酰丙酮酸。马来酰丙酮酸无需先进行顺,顺到顺,反异构化即可水解生成丙酮酸和马来酸。通过平板计数法检测了大量土壤,发现每克干土中含有10⁴至10⁶个能够在间羟基苯甲酸上生长的需氧芽孢形成菌。