Standerfer R J, Rittenberg M B, Chern C J, Templeton J W, Black J A
Biochem Genet. 1975 Jun;13(5-6):341-51. doi: 10.1007/BF00485819.
We have compared the solubility, kinetic, immunological, and electrophoretic properties of erythrocyte pyruvate kinase from normal dogs and Basenji dogs with congenital hemolytic anemia due to pyruvate kinase deficiency. Differences can be detected between the two enzymes by all methods. The enzyme from the affected animals has a greater solubility in ammonium sulfate. It has a lower Km for phosphoenolpyruvate, while the Km for ADP is increased. This enzyme is not inhibited by ATP or activated by fructose 1,6-diphosphate. The enzyme from the affected animals has none of the allosteric properties characteristic of the normal canine enzyme. No difference can be detected by enzyme inactivation with rabbit antiserum against human erythrocyte enzyme, but a slight spur is observed on comparison of the two enzymes by Ouchterlony immunodiffusion. The enzymes also differ in their electrophoretic mobilities on starch gel electrophoresis.
我们比较了正常犬和因丙酮酸激酶缺乏而患有先天性溶血性贫血的巴仙吉犬红细胞丙酮酸激酶的溶解性、动力学、免疫学和电泳特性。通过所有方法都能检测到这两种酶之间的差异。患病动物的酶在硫酸铵中的溶解度更高。它对磷酸烯醇丙酮酸的米氏常数较低，而对二磷酸腺苷的米氏常数增加。这种酶不受三磷酸腺苷抑制，也不被1,6-二磷酸果糖激活。患病动物的酶没有正常犬类酶所特有的别构性质。用兔抗人红细胞酶血清进行酶失活处理时，未检测到差异，但在双向免疫扩散法比较这两种酶时，观察到了轻微的沉淀线。这两种酶在淀粉凝胶电泳中的电泳迁移率也有所不同。
