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The Asc locus for resistance to Alternaria stem canker in tomato does not encode the enzyme aspartate carbamoyltransferase.

作者信息

Overduin B, Hogenhout S A, van der Biezen E A, Haring M A, Nijkamp H J, Hille J

机构信息

Department of Genetics, Vrije Universiteit Amsterdam, The Netherlands.

出版信息

Mol Gen Genet. 1993 Jul;240(1):43-8. doi: 10.1007/BF00276882.

DOI:10.1007/BF00276882
PMID:8101964
Abstract

The fungal disease resistance locus Alternaria stem canker (Asc) in tomato has been suggested to encode the enzyme aspartate carbamoyltransferase (ACTase). To test this hypothesis a segment of the tomato ACTase gene was amplified by the polymerase chain reaction (PCR) using degenerate primers. The PCR product obtained was subsequently used to isolate an ACTase cDNA clone. Restriction fragment length polymorphism (RFLP) linkage analysis showed that the ACTase gene and the Asc locus do not cosegregate. RFLP mapping positioned the ACTase gene on chromosome 11, while the Asc locus is located on chromosome 3. These results exclude the possibility that the ACTase protein is encoded by the Asc locus.

摘要

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Tomato resistance to Alternaria stem canker: localization in host genotypes and functional expression compared to non-host resistance.番茄对交链格孢茎溃疡病的抗性：与非寄主抗性相比，在寄主基因型中的定位和功能表达。
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The rudimentary gene of Drosophila melanogaster encodes four enzymic functions.果蝇的原始基因编码四种酶功能。
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