Butler W R, O'Connor S P, Yakrus M A, Smithwick R W, Plikaytis B B, Moss C W, Floyd M M, Woodley C L, Kilburn J O, Vadney F S
Division of Bacterial and Mycotic Diseases, Centers for Diseases Control and Prevention, Atlanta, Georgia 30333.
Int J Syst Bacteriol. 1993 Jul;43(3):539-48. doi: 10.1099/00207713-43-3-539.
A new slowly growing nonphotochromogenic Mycobacterium species of clinical importance is described. The biochemical characteristics of this organism were similar to those of Mycobacterium xenopi and members of the Mycobacterium avium complex. However, none of the strains reacted with commercially available genetic probes for the M. avium complex. The strains were resistant to most antituberculosis drugs. Multilocus enzyme electrophoresis revealed two original electrophoretic types, which was suggestive of new species. The strains contained alpha-, keto-, and dicarboxylic mycolates, as determined by thin-layer chromatography. A mycolic acid analysis by high-performance liquid chromatography revealed a chromatographic pattern similar to that of M. xenopi, but distinct from the patterns of previously described Mycobacterium species. Hexadecanoic and tuberculostearic acids were identified as the major cell wall fatty acids by gas-liquid chromatographic analysis; hexacosanoic acid was the major mycolic acid cleavage product, and 2-eicosanol was the major alcohol. Evaluation of the 16S rRNA sequence confirmed the phylogenetic position of the organism among the slowly growing Mycobacterium species. Cultures representing this new species have been deposited in the American Type Culture Collection as strains ATCC 51130 and ATCC 51131T (T = type strain). The name Mycobacterium celatum is proposed.
描述了一种具有临床重要性的新的缓慢生长的非光产色分枝杆菌菌种。该菌的生化特性与偶发分枝杆菌及鸟分枝杆菌复合群的成员相似。然而，这些菌株均未与市售的鸟分枝杆菌复合群基因探针发生反应。这些菌株对大多数抗结核药物耐药。多位点酶电泳显示出两种原始电泳类型，提示为新菌种。通过薄层色谱法测定，这些菌株含有α-、酮-和二羧酸分枝菌酸。高效液相色谱法进行的分枝菌酸分析显示出一种与偶发分枝杆菌相似但不同于先前描述的分枝杆菌菌种模式的色谱图。气液色谱分析鉴定出十六烷酸和结核硬脂酸为主要细胞壁脂肪酸；二十六烷酸为主要分枝菌酸裂解产物，2-二十醇为主要醇类。对16S rRNA序列的评估证实了该菌在缓慢生长的分枝杆菌菌种中的系统发育位置。代表该新菌种的培养物已作为ATCC 51130和ATCC 51131T菌株（T = 模式菌株）保藏于美国模式培养物集存库。提议将其命名为隐蔽分枝杆菌。
