Floyd M M, Guthertz L S, Silcox V A, Duffey P S, Jang Y, Desmond E P, Crawford J T, Butler W R
Division of AIDS, STD, and TB Laboratory Research, National Centers for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1996 Dec;34(12):2963-7. doi: 10.1128/jcm.34.12.2963-2967.1996.
Polyphasic taxonomic methods were employed to characterize a new species of slowly growing, nonpigmented mycobacteria. We propose the name Mycobacterium triplex sp. nov. for this new taxon. Conventional identification testing demonstrated a group of similar organisms that were geographically widespread in the United States. Commercially available nucleic-acid probes specific for the Mycobacterium avium complex were unreactive for these strains. High-performance liquid chromatography analysis of the mycolic acids revealed mycolate profiles that closely resembled Mycobacterium simiae. Comparative 16S rRNA sequence data confirmed the phylogenetic relationship of the strains with the slowly growing mycobacteria. Representative-type strains have been deposited in the American Type Culture Collection as strain ATCC 700071 [corrected].
采用多相分类方法对一种新的缓慢生长、无色素分枝杆菌进行了特征描述。我们提议将这个新分类单元命名为Mycobacterium triplex sp. nov.。传统的鉴定测试表明，在美国，有一组相似的生物体广泛分布于各地。针对鸟分枝杆菌复合群的市售核酸探针与这些菌株无反应。对分枝菌酸的高效液相色谱分析显示，其分枝菌酸谱与猿猴分枝杆菌非常相似。比较16S rRNA序列数据证实了这些菌株与缓慢生长分枝杆菌的系统发育关系。代表性菌株已作为ATCC 700071菌株保藏于美国模式培养物集存库[已校正]。
