Ludwig R, Matzku S, Rapp W
Clin Chim Acta. 1975 Nov 3;64(3):253-61. doi: 10.1016/0009-8981(75)90352-6.
Alpha1-Acid glycoprotein (AGP) was quantitated by an electroradioimmunoassay (ERIA) in in vivo neutralized gastric juice (nGJ) of 226 patients, including normals and patients with various gastic diseases. The accuracy of ERIA was tested by extraction and recovery experiments. A possible interference of nGJ constituents with the quantitation procedure was excluded. The mean AGP concentration in gastric juice of normals was 1.04 mu/ml (range 0.04-4.1 mu/ml). The concentration was significantly higher in the gastric cancer group (mean 31.6 mu/ml, p less than 0.01), in the group of chronic metaplastic gastritis (mean 5.7 mu/ml, p less than 0.01) and in the group of BII resections (mean 8.8 mu/ml, p less than 0.05). In 6 out of 12 nGJ samples with high AGP concentrations, a spur formation (Ouchterlony type III) was observed in double gel diffusion when compared to serum AGP. In 3 out of these 12 samples, the dilution curve in ERIA differed from the serum AGP dilution curve. These results indicate a difference in the antigenic properties of AGP in nGJ.
采用电放射免疫分析法（ERIA）对226例患者（包括正常人和患有各种胃部疾病的患者）体内中和后的胃液（nGJ）中的α1-酸性糖蛋白（AGP）进行了定量分析。通过提取和回收率实验对ERIA的准确性进行了测试。排除了nGJ成分对定量过程可能产生的干扰。正常人胃液中AGP的平均浓度为1.04μg/ml（范围为0.04 - 4.1μg/ml）。胃癌组（平均31.6μg/ml，p<0.01）、慢性化生胃炎组（平均5.7μg/ml，p<0.01）和BII切除组（平均8.8μg/ml，p<0.05）的浓度明显更高。在12份AGP浓度较高的nGJ样本中，有6份与血清AGP相比，在双向凝胶扩散试验中观察到了刺状物形成（奥克特洛尼III型）。在这12份样本中的3份中，ERIA中的稀释曲线与血清AGP稀释曲线不同。这些结果表明nGJ中AGP的抗原特性存在差异。
