Rapp W, Heim M, von Mikulicz-Radecki J G, Ludwig R
Klin Wochenschr. 1975 Feb 1;53(3):139-41. doi: 10.1007/BF01466718.
Gastric juice was neutralized (nGJ) in vivo by 80 ml of a phosphate buffer containing radiolabelled vitamin B12 as dilution indicator. Unprocessed nGJ was analyzed in the double gel diffusion technique for the presence of serum proteins using monospecific antisera. Alpha1-Acid glycoprotein (AGP) was found in a high incidence (36 out of 38 subjects) in nGJ of gastric cancer patients. AGP was also observed less frequently in nGJ of patients with Billroth II resections (6/15), metaplasia (11/52), gastric ulcer (3/24), chronic atrophic gastritis (2/26) and chronic gastritis (3/63). AGP was absent in the control group (0/21), in patients with surface gastritis (0/38) and in subjects with normal acid secretion (0/45). Immunochemical studies demonstrated no identity of AGP with human "gastrointestinal tumor associated antigens." In 7 out of 17 AGP positive samples immunochemical differences between gastric and serum AGP were observed.
通过80毫升含有放射性标记维生素B12作为稀释指示剂的磷酸盐缓冲液在体内中和胃液（nGJ）。使用单特异性抗血清，采用双向凝胶扩散技术分析未处理的nGJ中血清蛋白的存在情况。在胃癌患者的nGJ中，α1-酸性糖蛋白（AGP）的检出率很高（38名受试者中有36名）。在毕罗Ⅱ式切除术患者（6/15）、化生患者（11/52）、胃溃疡患者（3/24）、慢性萎缩性胃炎患者（2/26）和慢性胃炎患者（3/63）的nGJ中，AGP的检出频率也较低。对照组（0/21）、表浅性胃炎患者（0/38）和胃酸分泌正常的受试者（0/45）中未检测到AGP。免疫化学研究表明，AGP与人类“胃肠道肿瘤相关抗原”不同。在17份AGP阳性样本中的7份中，观察到胃AGP和血清AGP之间存在免疫化学差异。
