Mizukami H, Ohbayashi K, Kitamura Y, Ikenaga T
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Biol Pharm Bull. 1993 Apr;16(4):388-90. doi: 10.1248/bpb.16.388.
A simple and efficient method for identification of Duboisia leichhardtii F. Muell, D. myoporoides R. BR. and their interspecific hybrid was established by analyzing restriction fragment length polymorphism (RFLP) profiles using non-radioactive rice ribosomal DNA as a probe. This procedure was shown to be applicable to both fresh and dried samples, and also suitable for detection of somatic hybrid at the callus stage after protoplast fusion.
通过使用非放射性水稻核糖体DNA作为探针分析限制性片段长度多态性(RFLP)图谱,建立了一种简单有效的方法来鉴定莱氏澳洲茄(Duboisia leichhardtii F. Muell)、澳洲茄(D. myoporoides R. BR.)及其种间杂种。该方法适用于新鲜和干燥样本,也适用于检测原生质体融合后愈伤组织阶段的体细胞杂种。