Unger E R, Sung J H, Manivel J C, Chenggis M L, Blazar B R, Krivit W
Department of Pathology, Emory University, Atlanta, Georgia.
J Neuropathol Exp Neurol. 1993 Sep;52(5):460-70. doi: 10.1097/00005072-199309000-00004.
In five female bone marrow transplant (BMT) recipients of sex-mismatched donor marrow, Y-chromosome specific in situ hybridization was performed on formalin-fixed, paraffin-embedded sections of the medulla to detect the male donor marrow-derived cells. Y-chromosome-bearing cells (Y-cells), thereby donor-derived, were matched with leukocyte common antigen (LCA)-reactive cells in adjacent sections immunostained with anti-LCA antibody. Y-cells included mononuclear leukocytes (MNL) within the vessel lumen and infiltrating the perivascular space and parenchyma, and "perivascular cells." We have, therefore, concluded that donor marrow-derived MNL, though limited in number, do enter the normal-appearing brain and can transform to "perivascular cells" in human BMT recipients. It remains, however, to be confirmed whether MNL entering the normal adult CNS parenchyma transform to ramified microglia.
在5例接受性别不匹配供体骨髓移植（BMT）的女性患者中，对福尔马林固定、石蜡包埋的骨髓切片进行Y染色体特异性原位杂交，以检测男性供体骨髓来源的细胞。带有Y染色体的细胞（Y细胞），即供体来源的细胞，与用抗白细胞共同抗原（LCA）抗体免疫染色的相邻切片中的LCA反应性细胞相匹配。Y细胞包括血管腔内和浸润血管周围间隙及实质的单核白细胞（MNL），以及“血管周围细胞”。因此，我们得出结论，供体骨髓来源的MNL数量虽有限，但确实可进入外观正常的脑内，并在人类BMT受者中转化为“血管周围细胞”。然而，进入正常成人中枢神经系统实质的MNL是否会转化为分支状小胶质细胞仍有待证实。
