Kurachi H, Adachi H, Ohtsuka S, Morishige K, Amemiya K, Keno Y, Shimomura I, Tokunaga K, Miyake A, Matsuzawa Y
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Am J Physiol. 1993 Aug;265(2 Pt 1):E323-31. doi: 10.1152/ajpendo.1993.265.2.E323.
Ovariectomy (Ovx) of mice significantly increases the epidermal growth factor (EGF) concentration in the submandibular gland. To elucidate the role of this elevated EGF in obesity of Ovx mice, we examined the effects of sialoadenectomy (Sx) and anti-EGF rabbit antiserum administration on the body weight (BW) gain and carcass fat deposition in Ovx animals. Studies were performed in four groups of mice consisting of control, Ovx, Ovx+Sx, and Ovx+anti-EGF groups. Ovx increased the BW gain compared with the control animals, whereas Sx and anti-EGF significantly reduced it. Although the relative weights (weight ratio to BW) of the liver and kidney were not significantly changed by Ovx, Sx, or anti-EGF treatment of Ovx mice, the relative weights of mesenteric, parametrial, and subcutaneous fat tissues were increased in Ovx mice, and this increase was significantly reduced by Sx or anti-EGF administration. Ovx induced adipocyte hypertrophy, and this effect was eliminated by Sx and anti-EGF. Moreover, acyl-CoA synthetase mRNA level was increased by Ovx, and this increase was reduced by Sx and anti-EGF in mesenteric fat tissue. These findings suggest that elevation of EGF may play a role in the induction of obesity in Ovx mice.
切除小鼠卵巢（Ovx）会显著提高其下颌下腺中表皮生长因子（EGF）的浓度。为了阐明这种升高的EGF在去卵巢小鼠肥胖中的作用，我们研究了涎腺切除术（Sx）和给予抗EGF兔抗血清对去卵巢动物体重（BW）增加和胴体脂肪沉积的影响。实验在四组小鼠中进行，分别为对照组、去卵巢组、去卵巢+涎腺切除组和去卵巢+抗EGF组。与对照动物相比，去卵巢组的体重增加，而涎腺切除组和抗EGF组则显著降低了体重增加。虽然去卵巢、涎腺切除或对去卵巢小鼠进行抗EGF处理对肝脏和肾脏的相对重量（与体重的重量比）没有显著影响，但去卵巢小鼠肠系膜、子宫旁和皮下脂肪组织的相对重量增加，而涎腺切除或给予抗EGF可显著降低这种增加。去卵巢诱导脂肪细胞肥大，而涎腺切除和抗EGF可消除这种作用。此外，去卵巢会使肠系膜脂肪组织中的酰基辅酶A合成酶mRNA水平升高，而涎腺切除和抗EGF可降低这种升高。这些发现表明，EGF升高可能在去卵巢小鼠肥胖的诱导中起作用。
