Killgore G E, Kato H
Nosocomial Pathogens Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
J Clin Microbiol. 1994 Jun;32(6):1591-3. doi: 10.1128/jcm.32.6.1591-1593.1994.
An arbitrarily primed PCR (AP-PCR) assay was used to type Clostridium difficile isolates from a hospital outbreak of antibiotic-associated diarrhea. Forty-one isolates were separated into nine groups, with 66% falling into one group; no other group contained more than 10%. Comparison of AP-PCR grouping with that when the immunoblot technique was used showed agreement for 33 of 34 isolates typed by both techniques, and AP-PCR grouped seven isolates that were not typeable by immunoblotting.
采用任意引物聚合酶链反应（AP-PCR）分析法对一家医院抗生素相关性腹泻暴发中的艰难梭菌分离株进行分型。41株分离株被分为9组，其中66%属于同一组；其他组的分离株均不超过10%。将AP-PCR分型结果与免疫印迹技术的分型结果进行比较，结果显示，在两种技术均进行分型的34株分离株中，有33株结果一致，并且AP-PCR将7株无法用免疫印迹法分型的分离株进行了分组。
