Ring J, Enders B, Seiler F, Seifert J, Steininger J, Brendel W
Int Arch Allergy Appl Immunol. 1976;50(1):103-10. doi: 10.1159/000231486.
Sheep red blood cells were stabilized with sulfosalicylic acid and sensitized with purified horse-IgG by the action of glutardialdehyde. The cells were used in an indirect microhemmagglutination test with serum samples from 12 rabbits immunized with horse-IgG and complete Freund's adjuvant as well as from 10 control animals. 32 human serum samples were examined, 6 of them from patients with established horse protein allergy. The results were compared to those of a standard method of indirect hemagglutination using fresh human erythrocytes (group O Rh-), an active hemagglutination test against horse erythrocytes, Ouchterlony's immunodiffusion and skin tests with horse-IgG. The method proved to be very sensitive. The results correlated well with those of the other immunological techniques. The stable sensitized cells did not lose sensitivity after 1 year of storage at 4 degrees C.
绵羊红细胞用磺基水杨酸稳定，并通过戊二醛的作用用纯化的马IgG致敏。这些细胞用于间接微血凝试验，检测12只用马IgG和完全弗氏佐剂免疫的兔子以及10只对照动物的血清样本。检测了32份人血清样本，其中6份来自确诊对马蛋白过敏的患者。将结果与使用新鲜人红细胞（O型Rh-）的间接血凝标准方法、针对马红细胞的活性血凝试验、双向免疫扩散试验以及马IgG皮肤试验的结果进行比较。该方法证明非常灵敏。结果与其他免疫技术的结果相关性良好。稳定的致敏细胞在4℃储存1年后未丧失敏感性。
