Hambie E A, Larsen S A, Felker M, Jones W L, Feeley J C
J Clin Microbiol. 1977 Feb;5(2):167-71. doi: 10.1128/jcm.5.2.167-171.1977.
Two evaluations were carried out in this study. The first was a comparison of the standard tube test with the automated microtitration test for the detection of antibodies to Pseudomonas pseudomallei by the indirect hemagglutination method. Data from this comparison indicated that the tests were equivalent. The second evaluation consisted of reproducibility studies on two lots of pyruvic aldehyde-stabilized sensitized erythrocytes in comparison with freshly prepared sensitized erythrocytes in the automated microtitration test. The influence of different types of the microtitration plates used was also examined. Results indicated that the use of stabilized antigens is feasible, and these antigens offer the advantage of being ready for immediate use.
本研究进行了两项评估。第一项是通过间接血凝法，比较标准试管试验与自动微量滴定试验检测类鼻疽杆菌抗体的情况。该比较的数据表明这两种试验等效。第二项评估包括在自动微量滴定试验中，对两批丙酮酸醛稳定化致敏红细胞与新鲜制备的致敏红细胞进行重复性研究。同时还考察了所使用的不同类型微量滴定板的影响。结果表明使用稳定化抗原是可行的，并且这些抗原具有可立即使用的优点。
