蓝绿细菌集胞藻中亮氨酸的生物合成
Leucine biosynthesis in the blue-green bacterium Anacystis nidulans.
作者信息
Singer R A, Doolittle W F
出版信息
J Bacteriol. 1975 Nov;124(2):810-4. doi: 10.1128/jb.124.2.810-814.1975.
Abstract
Leucine-requiring auxotrophs of the unicellular blue-green bacterium Anacystis nidulans have been isolated. Extracts of these mutants were deficient in alpha-isopropylmalate synthetase (EC 4.1.3.12). In wild-type cells, this enzyme was subject to feedback inhibition by leucine. However, formation of the enzymes of leucine biosynthesis was little affected by exogenous leucine in either wild-type or mutant strains. Cultures of the latter subjected to extreme leucine deprivation showed no change in specific activity of beta-isopropylmalate isomerase (EC 4.2.1.33) and at most a 50% increase in the specific activity of beta-isopropylmalate dehydrogenase (EC 1.1.1.85). These results are compared with others bearing on the evolution of the control of amino acid biosynthesis in blue-green bacteria.
摘要
已分离出单细胞蓝细菌集胞藻（Anacystis nidulans）的亮氨酸营养缺陷型菌株。这些突变体的提取物缺乏α-异丙基苹果酸合成酶（EC 4.1.3.12）。在野生型细胞中，该酶受到亮氨酸的反馈抑制。然而，亮氨酸生物合成酶的形成在野生型或突变菌株中几乎都不受外源亮氨酸的影响。对后者进行极端亮氨酸剥夺处理的培养物中，β-异丙基苹果酸异构酶（EC 4.2.1.33）的比活性没有变化，β-异丙基苹果酸脱氢酶（EC 1.1.1.85）的比活性最多增加50%。将这些结果与其他有关蓝细菌氨基酸生物合成调控进化的结果进行了比较。
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