Macchia G, Massone A, Burroni D, Covacci A, Censini S, Rappuoli R
IRIS Immunobiological Research Institute Siena, Italy.
Mol Microbiol. 1993 Aug;9(3):645-52. doi: 10.1111/j.1365-2958.1993.tb01724.x.
Helicobacter pylori is a human pathogen that has been associated with gastritis, peptic ulcer and gastric carcinoma. The role of the direct action of H. pylori virulence factors and of the induction of autoreactive immunity in the development of chronic gastritis has not been clarified yet. Here we report the cloning and molecular characterization of a gene of H. pylori coding for a protein of 58 kDa, recognized by sera of patients affected by H. pylori-induced gastroduodenal diseases. This antigen is present in all the H. pylori strains tested and it belongs to the Hsp60 family of heat-shock proteins, with high homology with other bacterial and eukaryotic proteins of the same family. This class of homologous proteins has been implicated in the induction of autoimmune disorders in different systems. The presence in infected patients of anti-H. pylori Hsp60 antibodies, potentially cross-reactivity between human Hsp60 and a rabbit antiserum against H. pylori Hsp60 suggest that a role of this protein in gastroduodenal diseases is possible.
幽门螺杆菌是一种人类病原体，与胃炎、消化性溃疡和胃癌有关。幽门螺杆菌毒力因子的直接作用以及自身反应性免疫的诱导在慢性胃炎发展中的作用尚未阐明。在此，我们报告了幽门螺杆菌一个基因的克隆及分子特征，该基因编码一种58 kDa的蛋白质，受幽门螺杆菌诱导的胃十二指肠疾病患者的血清可识别该蛋白。这种抗原存在于所有测试的幽门螺杆菌菌株中，它属于热休克蛋白的Hsp60家族，与同一家族的其他细菌和真核蛋白具有高度同源性。这类同源蛋白已被证明在不同系统中可诱导自身免疫性疾病。感染患者体内存在抗幽门螺杆菌Hsp60抗体、人Hsp60与兔抗幽门螺杆菌Hsp60抗血清之间可能存在的交叉反应，表明该蛋白在胃十二指肠疾病中可能发挥作用。
