残余的游离钙并不负责促进神经递质的释放。
Residual free calcium is not responsible for facilitation of neurotransmitter release.
作者信息
Blundon J A, Wright S N, Brodwick M S, Bittner G D
机构信息
Department of Zoology, University of Texas, Austin 78712.
出版信息
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9388-92. doi: 10.1073/pnas.90.20.9388.
Abstract
An increase in internal free calcium ([Ca2+]i) in the presynaptic terminal is often assumed to directly produce facilitation of neurotransmitter release. Using a Ca(2+)-activated potassium conductance as a bioassay for free [Ca2+]i in the presynaptic terminal of the crayfish (Procambarus clarkii) opener neuromuscular junction, we now demonstrate that free [Ca2+]i has a decay time constant (tau) of 1-4 msec, whereas facilitation of neurotransmitter release has a decay tau of 7-43 msec. In addition, facilitation of neurotransmitter release can be markedly different at times when free [Ca2+]i values and presynaptic membrane voltages are equal. We conclude that free [Ca2+]i in the presynaptic terminal is not directly responsible for facilitation of neurotransmitter release. Our data suggest that facilitation results from bound Ca2+ or some long-lived consequence of bound Ca2+.
摘要
通常认为，突触前终末内的游离钙（[Ca2+]i）增加会直接导致神经递质释放的易化。我们利用一种钙激活钾电导作为小龙虾（克氏原螯虾）开肌神经肌肉接头突触前终末游离[Ca2+]i的生物测定方法，现证明游离[Ca2+]i的衰减时间常数（tau）为1 - 4毫秒，而神经递质释放的易化具有7 - 43毫秒的衰减tau。此外，当游离[Ca2+]i值和突触前膜电压相等时，神经递质释放的易化在不同时间可能会有显著差异。我们得出结论，突触前终末内的游离[Ca2+]i并非直接导致神经递质释放的易化。我们的数据表明，易化是由结合钙或结合钙的某些长时效应引起的。
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本文引用的文献
1
An analysis of facilitation of transmitter release at the neuromuscular junction of the frog.对青蛙神经肌肉接头处递质释放促进作用的分析。
J Physiol. 1967 Dec;193(3):679-94. doi: 10.1113/jphysiol.1967.sp008388.
2
The components of membrane conductance in the giant axon of Loligo.枪乌贼巨大轴突膜电导的组成成分。
J Physiol. 1952 Apr;116(4):473-96. doi: 10.1113/jphysiol.1952.sp004718.
3
Presynaptic calcium diffusion and the time courses of transmitter release and synaptic facilitation at the squid giant synapse.鱿鱼巨大突触处的突触前钙扩散、递质释放的时间进程及突触易化
J Neurosci. 1983 Jun;3(6):1263-9. doi: 10.1523/JNEUROSCI.03-06-01263.1983.
4
An examination of the residual calcium theory for facilitation of transmitter release.对递质释放易化的残余钙理论的检验。
Brain Res. 1981 Apr 6;210(1-2):431-6. doi: 10.1016/0006-8993(81)90922-7.
5
Co-operative action a calcium ions in transmitter release at the neuromuscular junction.钙离子在神经肌肉接头处递质释放中的协同作用。
J Physiol. 1967 Nov;193(2):419-32. doi: 10.1113/jphysiol.1967.sp008367.
6
The role of calcium in neuromuscular facilitation.钙在神经肌肉易化中的作用。
J Physiol. 1968 Mar;195(2):481-92. doi: 10.1113/jphysiol.1968.sp008469.
7
On facilitation of transmitter release at the toad neuromuscular junction.关于蟾蜍神经肌肉接头处递质释放的促进作用。
J Physiol. 1974 Jun;239(3):657-75. doi: 10.1113/jphysiol.1974.sp010588.
8
The biophysical pharmacology of calcium-dependent acetylcholine secretion.钙依赖性乙酰胆碱分泌的生物物理药理学
Pharmacol Rev. 1985 Mar;37(1):81-132.
9
Decline in calcium cooperativity as the basis of facilitation at the squid giant synapse.钙协同作用的降低作为鱿鱼巨突触易化作用的基础。
J Neurosci. 1986 Mar;6(3):782-9. doi: 10.1523/JNEUROSCI.06-03-00782.1986.
10
Intraterminal injection of synapsin I or calcium/calmodulin-dependent protein kinase II alters neurotransmitter release at the squid giant synapse.在乌贼巨大突触处，向突触小体注射突触结合蛋白I或钙/钙调蛋白依赖性蛋白激酶II会改变神经递质的释放。
Proc Natl Acad Sci U S A. 1985 May;82(9):3035-9. doi: 10.1073/pnas.82.9.3035.
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