Beylot M, David F, Brunengraber H
INSERM U197, Lyon, France.
Anal Biochem. 1993 Aug 1;212(2):532-6. doi: 10.1006/abio.1993.1364.
We present a simple technique for determining the 13C-labeling pattern of glutamate by gas chromatography-mass spectrometry. Glutamate is derivatized with dimethylformamide dimethyl acetal (Methyl-8R). The dimethylaminomethylene methyl ester derivative of glutamate yields fragment ions that allow calculation of 13C enrichment on each carbon. The technique was tested by analyzing glutamate from rat livers perfused with various 13C tracers. The labeling patterns obtained agreed with theoretical calculations or patterns reported with 14C and 13C tracers.
我们介绍了一种通过气相色谱 - 质谱法测定谷氨酸13C标记模式的简单技术。谷氨酸用二甲基甲酰胺二甲基缩醛（Methyl - 8R）进行衍生化。谷氨酸的二甲基氨基亚甲基甲酯衍生物产生的碎片离子可用于计算每个碳原子上的13C富集情况。通过分析用各种13C示踪剂灌注的大鼠肝脏中的谷氨酸对该技术进行了测试。获得的标记模式与理论计算结果或用14C和13C示踪剂报道的模式一致。
