Elazar Z, Orci L, Ostermann J, Amherdt M, Tanigawa G, Rothman J E
Program in Cellular Biochemistry and Biophysics, Memorial Sloan Kettering Cancer Center, New York 10021.
J Cell Biol. 1994 Feb;124(4):415-24. doi: 10.1083/jcb.124.4.415.
The coat proteins required for budding COP-coated vesicles from Golgi membranes, coatomer and ADP-ribosylation factor (ARF) protein, are shown to be required to reconstitute the orderly process of transport between Golgi cisternae in which fusion of transport vesicles begins only after budding ends. When either coat protein is omitted, fusion is uncoupled from budding-donor and acceptor compartments pair directly without an intervening vesicle. Coupling may therefore results from the sequestration of fusogenic membrane proteins into assembling coated vesicles that are only exposed when the coat is removed after budding is complete. This mechanism of coupling explains the phenomenon of "retrograde transport" triggered by uncouplers such as the drug brefeldin A.
从高尔基体膜上出芽形成COP被膜小泡所需的衣被蛋白，即衣被蛋白复合体和ADP核糖基化因子（ARF）蛋白，被证明是重建高尔基体潴泡间有序运输过程所必需的，在这个过程中，运输小泡的融合仅在出芽结束后才开始。当省略任何一种衣被蛋白时，融合与出芽直接解偶联，供体和受体区室直接配对，无需中间小泡。因此，偶联可能是由于促融合膜蛋白被隔离到正在组装的被膜小泡中，这些小泡只有在出芽完成后衣被被去除时才会暴露出来。这种偶联机制解释了由诸如药物布雷菲德菌素A等解偶联剂引发的“逆行运输”现象。
