Stamnes M A, Craighead M W, Hoe M H, Lampen N, Geromanos S, Tempst P, Rothman J E
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):8011-5. doi: 10.1073/pnas.92.17.8011.
We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad repeats found in coiled coils. When the yeast p24 isoform, yp24A, is knocked out in a strain defective for vesicle fusion, a dramatic reduction in the accumulation of transport vesicles is observed. Together, these results indicate a role for this protein family in the budding of coatamer-coated and other species of coated vesicles.
我们从高尔基体衍生的包被蛋白包被小泡中分离出一种主要的整合膜蛋白。这种24 kDa的蛋白，即p24，定义了一个整合膜蛋白家族，其同源物存在于酵母和人类中。除了序列相似性外，所有p24家族成员都含有一个基序，该基序具有在卷曲螺旋中发现的特征性七肽重复序列。当酵母p24异构体yp24A在一个囊泡融合缺陷的菌株中被敲除时，观察到运输小泡的积累显著减少。这些结果共同表明该蛋白家族在包被蛋白包被的小泡和其他种类的包被小泡出芽过程中发挥作用。
