Gordon V, Parry S, Bellamy K, Osborne R
Department of Veterinary Pathology, Glasgow University Veterinary School, UK.
J Virol Methods. 1993 Dec 31;45(3):247-57. doi: 10.1016/0166-0934(93)90111-4.
The aim of this study was to develop a standardised technique for assessing the virucidal activity of commercial disinfectants against human immunodeficiency virus (HIV). In the absence of any model procedure for HIV a protocol based on German DVV guidelines was developed. A major difficulty associated with such studies is the cytotoxic effect of the biocide on the target cells used in infectivity assays. This problem is most commonly overcome by dilution of the virus-disinfectant mixture, however, this requires high titre (> or = 10(7) TCID50) virus which is difficult to achieve with HIV. We employed a simple washing technique which effectively removed cytotoxicity while retaining infectivity. Incorporated into a standard suspension test, this method supported by virus isolation procedures was sensitive and reproducible. The reliability of the procedure was confirmed by evaluating the efficacy of some commercially available cidals which were known to be cytotoxic; namely two instrument disinfectants, Sactimed-I-Steril, an aldehyde based product, Sactimed-I-Sinald a guanide/quaternary-ammonium combination, and Levermed, an alcohol based hand disinfectant.
本研究的目的是开发一种标准化技术，用于评估商用消毒剂对人类免疫缺陷病毒（HIV）的杀病毒活性。由于缺乏针对HIV的任何标准程序，因此基于德国消毒协会（DVV）指南制定了一项方案。此类研究面临的一个主要困难是杀菌剂对用于感染性测定的靶细胞具有细胞毒性作用。解决这个问题最常用的方法是稀释病毒 - 消毒剂混合物，然而，这需要高滴度（≥10⁷ TCID₅₀）的病毒，而这对于HIV来说很难实现。我们采用了一种简单的洗涤技术，该技术在保留感染性的同时有效地消除了细胞毒性。将该技术纳入标准悬浮试验中，由病毒分离程序支持的这种方法灵敏且可重复。通过评估一些已知具有细胞毒性的市售杀菌剂的功效，证实了该程序的可靠性；即两种器械消毒剂，醛类产品Sactimed - I - Steril、胍/季铵化合物组合的Sactimed - I - Sinald，以及酒精类手部消毒剂Levermed。
