Moskophidis D, Battegay M, Bruendler M A, Laine E, Gresser I, Zinkernagel R M
Institute of Experimental Immunology, University of Zurich, Switzerland.
J Virol. 1994 Mar;68(3):1951-5. doi: 10.1128/JVI.68.3.1951-1955.1994.
The susceptibility to alpha/beta interferon (IFN-alpha/beta) or to gamma interferon (IFN-gamma) of various lymphocytic choriomeningitis virus (LCMV) strains was evaluated in C57BL/6 mice and in various cell lines. Anti-IFN-gamma treatment in vivo revealed that the LCMV strains Armstrong, Aggressive, and WE were most susceptible to IFN-gamma whereas Traub, Cl 13-Armstrong, and Docile were resistant. The same pattern of susceptibility to recombinant IFN-gamma was observed in vitro. In vivo treatment with anti-IFN-alpha/beta showed a sizeable increase in replication of Aggressive, Armstrong, and WE; effects were less pronounced for Docile, Cl 13-Armstrong, or Traub. Correspondingly, WE, Armstrong, and Aggressive were all relatively sensitive to purified IFN-alpha/beta in vitro, and Cl 13-Armstrong, Docile, and Traub were more resistant. Overall, there was a good correlation between the capacity of LCMV strains to establish a persistent infection in adult immunocompetent mice and their relative resistance to IFN-gamma and IFN-alpha/beta.
在C57BL/6小鼠和各种细胞系中评估了不同淋巴细胞性脉络丛脑膜炎病毒（LCMV）毒株对α/β干扰素（IFN-α/β）或γ干扰素（IFN-γ）的敏感性。体内抗IFN-γ治疗显示，LCMV毒株阿姆斯特朗、侵袭性毒株和WE对IFN-γ最为敏感，而特劳布、Cl 13-阿姆斯特朗和温顺毒株则具有抗性。在体外也观察到了对重组IFN-γ的相同敏感性模式。体内抗IFN-α/β治疗显示，侵袭性毒株、阿姆斯特朗毒株和WE的复制有相当大的增加；对温顺毒株、Cl 13-阿姆斯特朗毒株或特劳布毒株的影响则不太明显。相应地，WE、阿姆斯特朗毒株和侵袭性毒株在体外对纯化的IFN-α/β都相对敏感，而Cl 13-阿姆斯特朗毒株、温顺毒株和特劳布毒株更具抗性。总体而言，LCMV毒株在成年免疫活性小鼠中建立持续感染的能力与其对IFN-γ和IFN-α/β的相对抗性之间存在良好的相关性。
