Schmidt E E, MacDonald I C, Groom A C
Department of Medical Biophysics, University of Western Ontario, London, Canada.
Scanning Microsc. 1993 Jun;7(2):613-28.
Splenic microcorrosion casts prepared using minimal volumes of material show that most of the flow passes through the region bordering the white pulp. However, the nature of these microcirculatory pathways has received little attention. We have studied these in dog, cat, rat, mouse, and normal versus diseased human spleens. In all 5 species, a marginal sinus (MS) of anastomosing vascular spaces 5-10 microns thick lies between the white pulp and marginal zone (MZ). The morphology differs between species and the MS is absent in immune thrombocytopenia. The MS fills by circumferential flow before blood passes outward to the MZ. Many capillaries supply the MS and MZ, their arrangement and degree of branching differing among species. Capillaries never terminate within the reticulum of the white pulp. In immune thrombocytopenia, marked vascular hyperplasia occurs within white pulp and MZ. The perimarginal cavernous sinus plexus (PMCS), found in human, dog and rat, comprises large flattened spaces up to 300 microns x 1000 microns in area and 30-100 microns thick. It lies between the MZ and red pulp or directly adjacent to white pulp, and receives flow principally via the MZ. In sinusal spleens, the MS, MZ and PMCS are drained by open-ended venous sinuses. In non-sinusal spleens, the MS and MZ are drained by pulp venules. Approximately 90% of the splenic inflow passes through the region bordering the white pulp, bypassing the filtration beds of the red pulp. This suggests that immunologic functions of the spleen take precedence over the filtration of blood cellular elements in the red pulp.
使用最少材料制备的脾脏微腐蚀铸型显示，大部分血流通过与白髓相邻的区域。然而，这些微循环途径的性质很少受到关注。我们在狗、猫、大鼠、小鼠以及正常和患病的人类脾脏中对其进行了研究。在所有这5个物种中，白髓和边缘区（MZ）之间存在一个由5-10微米厚的吻合血管腔隙组成的边缘窦（MS）。不同物种之间其形态有所不同，免疫性血小板减少症中不存在MS。在血液向外流向MZ之前，MS通过圆周流充盈。许多毛细血管供应MS和MZ，它们的排列和分支程度在不同物种之间有所不同。毛细血管从不终止于白髓的网状组织内。在免疫性血小板减少症中，白髓和MZ内会出现明显的血管增生。在人类、狗和大鼠中发现的边缘海绵窦丛（PMCS），由面积达300微米×1000微米、厚度为30-100微米的大扁平腔隙组成。它位于MZ和红髓之间或直接与白髓相邻，主要通过MZ接收血流。在窦状脾脏中，MS、MZ和PMCS由开口的静脉窦引流。在非窦状脾脏中，MS和MZ由脾小静脉引流。大约90%的脾脏流入血流通过与白髓相邻的区域，绕过红髓的滤过床。这表明脾脏的免疫功能优先于红髓中血细胞成分的滤过。
