Yamamoto I
Biochem Pharmacol. 1985 Aug 1;34(15):2713-20. doi: 10.1016/0006-2952(85)90572-6.
Male ddY mice were given one injection of (1) mercury (mercuric chloride) simultaneously with various doses of selenium (sodium selenite), (2) mercury alone, or (3) various doses of selenium alone. The interaction between mercury and selenium in the liver and kidneys at 1, 5, 24, 120, and 240 hr after administration was investigated. The concentrations of mercury in the liver of mice receiving mercury and selenium simultaneously were higher than those after administration of mercury alone, while the concentrations of mercury in the kidney decreased markedly over a 1-120 hr period after administration, depending on the dose of selenium administered simultaneously with mercury. Clearly, selenium had a different effect on the accumulation of mercury in the liver and kidneys. Subcellular distribution studies revealed that mercury and selenium which were administered simultaneously were incorporated into the crude nuclear and mitochondrial fractions as stable complexes. The transport of these complexes to the kidneys seems to be limited. In addition, gel filtration of supernatant fractions of liver and kidney through a Sephadex G-75 column indicated that the proportion of mercury bound to metallothionein fraction decreased depending on the dose of selenium administered simultaneously with the mercury. This reduction was attributed to the decreased synthesis of mercury-thionein due to a reduction in the activity of Hg2+ which results from binding between mercury and selenium in the cells.
给雄性ddY小鼠注射一次：(1) 汞（氯化汞）并同时给予不同剂量的硒（亚硒酸钠），(2) 单独注射汞，或(3) 单独注射不同剂量的硒。研究给药后1、5、24、120和240小时肝脏和肾脏中汞与硒之间的相互作用。同时接受汞和硒的小鼠肝脏中的汞浓度高于单独注射汞后的浓度，而给药后1至120小时内，肾脏中的汞浓度根据与汞同时给予的硒剂量显著降低。显然，硒对肝脏和肾脏中汞的积累有不同影响。亚细胞分布研究表明，同时给予的汞和硒以稳定复合物的形式掺入粗核和线粒体部分。这些复合物向肾脏的转运似乎受到限制。此外，通过Sephadex G - 75柱对肝脏和肾脏上清液部分进行凝胶过滤表明，与金属硫蛋白部分结合的汞比例根据与汞同时给予的硒剂量而降低。这种降低归因于细胞中汞与硒结合导致Hg2+活性降低，从而使汞 - 硫蛋白的合成减少。
