Stickland N C
Stain Technol. 1975 Jul;50(4):255-64. doi: 10.3109/10520297509117068.
Nine different fixatives (Carnoy's, Susa, Baker's formalin, 5% formalin, 10% formalin, 10% formol saline, Bouin, Zenker, and 2.5% gluta-aldehyde) were compared by two methods. Gelatin-albumin gels were used to study volume changes after fixation and after various stages of subsequent processing. The appearance and hardness of the gels were also noted. The fixatives either shrunk or swelled the gels, but dehydration and clearing shrunk the gels in all cases. Samples of muscle tissue from one location in beef longissimus dorsi muscle were also placed in the different fixatives and processed. Various features were noted for each fixative, including the ease with which the paraffin wax blocks were cut and the staining ability of the sections in Mallory's triple stain. The diameters of the muscle fibers were measured from transverse sections of these samples and compared with the mean diameter of muscle fibers in a frozen unfixed section of muscle tissue. It was found that the fixatives had the same shrinkage effects on both the gels and the muscle samples. Analysis of variance tests showed that the various fixatives caused different degrees of shrinkage. Statistical details are given for the amounts of shrinkage caused by each fixative. Both the general histological picture and the amount of shrinkage were considered when deciding the best fixative. Carnoy was found to be the best of the fixatives investigated.
通过两种方法比较了九种不同的固定剂（卡诺固定液、苏萨固定液、贝克福尔马林、5%福尔马林、10%福尔马林、10%甲醛生理盐水、布因固定液、岑克尔固定液和2.5%戊二醛）。使用明胶-白蛋白凝胶研究固定后及后续处理各阶段后的体积变化。还记录了凝胶的外观和硬度。固定剂使凝胶收缩或膨胀，但在所有情况下脱水和透明处理都会使凝胶收缩。取自牛肉背最长肌一个部位的肌肉组织样本也置于不同的固定剂中进行处理。记录了每种固定剂的各种特征，包括石蜡块的切片难易程度以及切片在马洛里三色染色中的染色能力。从这些样本的横切片中测量肌肉纤维的直径，并与肌肉组织冷冻未固定切片中肌肉纤维的平均直径进行比较。发现固定剂对凝胶和肌肉样本具有相同的收缩作用。方差分析测试表明，各种固定剂导致不同程度的收缩。给出了每种固定剂引起的收缩量的统计细节。在确定最佳固定剂时，综合考虑了一般组织学图像和收缩量。结果发现，在所研究的固定剂中，卡诺固定液是最佳的。
