Tamaki Y, Kawamoto E, Araie M, Eguchi S, Fujii H
Eye Clinic, Tokyo Metropolitan Geriatric Hospital, Japan.
Nippon Ganka Gakkai Zasshi. 1994 Feb;98(2):162-8.
We have developed an apparatus using the laser speckle phenomenon for noninvasive two-dimensional measurements of the fundus microcirculation. The normalized blur of the speckle pattern (NB value) was adopted as an index of blood flow velocity in the tissue. The present study investigated whether the NB value can be also used as a quantitative index of the blood flow rate in the tissue. In experimental animals, an injection of a lethal dose of pentobarbital causes rapid reduction in the ocular perfusion pressure (OPP), which linearly correlates with blood flow rate in the ocular tissue. The NB in rabbit optic nerve head (ONH) tissue showed a linear correlation (r = 0.98, p < 0.001) with the OPP after injection of a lethal dose of pentobarbital, which suggested that the NB value parallels the blood flow rate in the ONH tissue. At various levels of OPP, the choroidal NB showed a good correlation (r = 0.60, p < 0.001) with the choroidal blood flow rate measured with microsphere technique. These results indicated that the NB value can be used as a quantitative index not only for the blood flow velocity but also for the blood flow rate in the tissue.
我们开发了一种利用激光散斑现象对眼底微循环进行无创二维测量的仪器。散斑图案的归一化模糊度（NB值）被用作组织中血流速度的指标。本研究调查了NB值是否也可作为组织中血流速率的定量指标。在实验动物中，注射致死剂量的戊巴比妥会导致眼灌注压（OPP）迅速降低，而眼灌注压与眼组织中的血流速率呈线性相关。在注射致死剂量的戊巴比妥后，兔视神经乳头（ONH）组织中的NB与OPP呈线性相关（r = 0.98，p < 0.001），这表明NB值与ONH组织中的血流速率平行。在不同水平的OPP下，脉络膜NB与用微球技术测量的脉络膜血流速率具有良好的相关性（r = 0.60，p < 0.001）。这些结果表明，NB值不仅可以用作组织中血流速度的定量指标，还可以用作组织中血流速率的定量指标。
