空肠弯曲菌四环素抗性决定子Tet(O)推定的GTP结合结构域中Asn-128突变改变的影响
Effect of mutational alteration of Asn-128 in the putative GTP-binding domain of tetracycline resistance determinant Tet(O) from Campylobacter jejuni.
作者信息
Grewal J, Manavathu E K, Taylor D E
机构信息
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
出版信息
Antimicrob Agents Chemother. 1993 Dec;37(12):2645-9. doi: 10.1128/AAC.37.12.2645.
Abstract
The deduced amino acid sequence of Campylobacter jejuni Tet(O), cloned in Escherichia coli, has shown that it contains the five highly conserved sequences of the GTP-binding domain found in other GTPases. Asn-128 belongs to the G4 motif of such a domain and is involved in hydrogen bonding with the guanine ring of the nucleotide. Substitution of Asn-128 by 11 other amino acids resulted in a decrease in tetracycline resistance, indicating that tetracycline resistance conferred by Tet(O) is related to GTP binding. The effect of the mutations on the GTP-binding domain is discussed with the EF-Tu-GDP complex as a model.
摘要
空肠弯曲杆菌Tet(O)在大肠杆菌中克隆后的推导氨基酸序列表明，它含有在其他GTP酶中发现的GTP结合结构域的五个高度保守序列。Asn-128属于该结构域的G4基序，参与与核苷酸鸟嘌呤环的氢键形成。用其他11种氨基酸取代Asn-128导致四环素抗性降低，表明Tet(O)赋予的四环素抗性与GTP结合有关。以EF-Tu-GDP复合物为模型讨论了这些突变对GTP结合结构域的影响。
相似文献
1
Effect of mutational alteration of Asn-128 in the putative GTP-binding domain of tetracycline resistance determinant Tet(O) from Campylobacter jejuni.空肠弯曲菌四环素抗性决定子Tet(O)推定的GTP结合结构域中Asn-128突变改变的影响
Antimicrob Agents Chemother. 1993 Dec;37(12):2645-9. doi: 10.1128/AAC.37.12.2645.
2
Molecular studies on the mechanism of tetracycline resistance mediated by Tet(O).关于Tet(O)介导的四环素抗性机制的分子研究
Antimicrob Agents Chemother. 1990 Jan;34(1):71-7. doi: 10.1128/AAC.34.1.71.
3
Incidence of antibiotic resistance in Campylobacter jejuni isolated in Alberta, Canada, from 1999 to 2002, with special reference to tet(O)-mediated tetracycline resistance.1999年至2002年在加拿大艾伯塔省分离出的空肠弯曲菌中抗生素耐药性的发生率，特别提及tet(O)介导的四环素耐药性。
Antimicrob Agents Chemother. 2004 Sep;48(9):3442-50. doi: 10.1128/AAC.48.9.3442-3450.2004.
4
Role of the plasmid-encoded tet(O) gene in tetracycline-resistant clinical isolates of Campylobacter jejuni and Campylobacter coli.质粒编码的tet(O)基因在空肠弯曲菌和结肠弯曲菌耐四环素临床分离株中的作用。
J Med Microbiol. 2007 Jun;56(Pt 6):833-837. doi: 10.1099/jmm.0.47103-0.
5
Effects of orally administered tetracycline on the intestinal community structure of chickens and on tet determinant carriage by commensal bacteria and Campylobacter jejuni.口服四环素对鸡肠道菌群结构以及共生细菌和空肠弯曲菌携带四环素决定簇的影响。
Appl Environ Microbiol. 2005 Oct;71(10):5865-72. doi: 10.1128/AEM.71.10.5865-5872.2005.
6
Tetracycline resistance of Australian Campylobacter jejuni and Campylobacter coli isolates.澳大利亚空肠弯曲菌和结肠弯曲菌分离株的四环素耐药性
J Antimicrob Chemother. 2005 Apr;55(4):452-60. doi: 10.1093/jac/dki040. Epub 2005 Mar 2.
7
16S rRNA mutations that confer tetracycline resistance in Helicobacter pylori decrease drug binding in Escherichia coli ribosomes.幽门螺杆菌中赋予四环素抗性的16S rRNA突变会降低药物在大肠杆菌核糖体中的结合。
J Bacteriol. 2005 Jun;187(11):3708-12. doi: 10.1128/JB.187.11.3708-3712.2005.
8
The tetracycline resistance protein Tet(o) perturbs the conformation of the ribosomal decoding centre.四环素抗性蛋白Tet(o)扰乱核糖体解码中心的构象。
Mol Microbiol. 2002 Sep;45(6):1463-72. doi: 10.1046/j.1365-2958.2002.03115.x.
9
Novel plasmid conferring kanamycin and tetracycline resistance in the turkey-derived Campylobacter jejuni strain 11601MD.在源自火鸡的空肠弯曲菌菌株11601MD中赋予卡那霉素和四环素抗性的新型质粒。
Plasmid. 2016 Jul;86:32-37. doi: 10.1016/j.plasmid.2016.06.001. Epub 2016 Jun 4.
10
New tetracycline resistance determinants coding for ribosomal protection in streptococci and nucleotide sequence of tet(T) isolated from Streptococcus pyogenes A498.编码链球菌核糖体保护的新型四环素抗性决定簇及从化脓性链球菌A498分离出的tet(T)的核苷酸序列。
Antimicrob Agents Chemother. 1997 Jan;41(1):112-6. doi: 10.1128/AAC.41.1.112.
引用本文的文献
1
Investigations on genomic, topological and structural properties of diguanylate cyclases involved in biofilm signalling using techniques: Promising drug targets in combating cholera.利用相关技术对参与生物膜信号传导的二鸟苷酸环化酶的基因组、拓扑结构和结构特性进行的研究：对抗霍乱的潜在药物靶点。
Curr Res Struct Biol. 2025 Apr 9;9:100166. doi: 10.1016/j.crstbi.2025.100166. eCollection 2025 Jun.
2
Ribosomal protection proteins and their mechanism of tetracycline resistance.核糖体保护蛋白及其四环素抗性机制。
Antimicrob Agents Chemother. 2003 Dec;47(12):3675-81. doi: 10.1128/AAC.47.12.3675-3681.2003.
3
Mechanism of Tet(O)-mediated tetracycline resistance.Tet(O)介导的四环素耐药机制。
EMBO J. 2003 Feb 17;22(4):945-53. doi: 10.1093/emboj/cdg093.
4
Host mutations (miaA and rpsL) reduce tetracycline resistance mediated by Tet(O) and Tet(M).宿主突变（miaA和rpsL）降低了由Tet(O)和Tet(M)介导的四环素抗性。
Antimicrob Agents Chemother. 1998 Jan;42(1):59-64. doi: 10.1128/AAC.42.1.59.
5
Glycylcyclines bind to the high-affinity tetracycline ribosomal binding site and evade Tet(M)- and Tet(O)-mediated ribosomal protection.甘氨酰环素类药物与高亲和力的四环素核糖体结合位点结合，并能逃避Tet(M)和Tet(O)介导的核糖体保护。
Antimicrob Agents Chemother. 1996 Sep;40(9):2226-8. doi: 10.1128/AAC.40.9.2226.
6
Tetracycline resistance mediated by ribosomal protection.核糖体保护介导的四环素耐药性。
Antimicrob Agents Chemother. 1996 Jan;40(1):1-5. doi: 10.1128/AAC.40.1.1.
7
Tet(M)-promoted release of tetracycline from ribosomes is GTP dependent.Tet(M)促进四环素从核糖体上释放是依赖鸟苷三磷酸（GTP）的。
J Bacteriol. 1996 Jun;178(11):3246-51. doi: 10.1128/jb.178.11.3246-3251.1996.
本文引用的文献
1
Heterogeneity of tetracycline resistance determinants in Streptococcus.链球菌中四环素抗性决定因素的异质性
J Bacteriol. 1982 Mar;149(3):995-1004. doi: 10.1128/jb.149.3.995-1004.1982.
2
Transport of the lipophilic analog minocycline differs from that of tetracycline in susceptible and resistant Escherichia coli strains.在敏感和耐药的大肠杆菌菌株中，亲脂性类似物米诺环素的转运与四环素不同。
Antimicrob Agents Chemother. 1982 Nov;22(5):791-9. doi: 10.1128/AAC.22.5.791.
3
Homologies between signal transducing G proteins and ras gene products.信号转导G蛋白与ras基因产物之间的同源性。
Science. 1984 Nov 16;226(4676):860-2. doi: 10.1126/science.6436980.
4
Photoincorporation of tetracycline into Escherichia coli ribosomes. Identification of the major proteins photolabeled by native tetracycline and tetracycline photoproducts and implications for the inhibitory action of tetracycline on protein synthesis.四环素掺入大肠杆菌核糖体。天然四环素和四环素光产物光标记的主要蛋白质的鉴定及其对四环素抑制蛋白质合成作用的意义。
Biochemistry. 1983 Jan 18;22(2):359-68. doi: 10.1021/bi00271a020.
5
Rapid and efficient site-specific mutagenesis without phenotypic selection.无需表型筛选的快速高效位点特异性诱变。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92. doi: 10.1073/pnas.82.2.488.
6
A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory protein.一种改变延伸因子Tu（一种GTP调节蛋白）核苷酸特异性的突变。
J Biol Chem. 1987 Sep 25;262(27):13081-5.
7
Homology of the TetM with translational elongation factors: implications for potential modes of tetM-conferred tetracycline resistance.TetM与翻译延伸因子的同源性：对tetM介导的四环素抗性潜在模式的影响。
Nucleic Acids Res. 1988 Feb 11;16(3):1218. doi: 10.1093/nar/16.3.1218.
8
GTP-binding domain: three consensus sequence elements with distinct spacing.GTP结合结构域：具有不同间隔的三个共有序列元件。
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1814-8. doi: 10.1073/pnas.84.7.1814.
9
Streptococcal tetracycline resistance mediated at the level of protein synthesis.链球菌对四环素的耐药性在蛋白质合成水平介导。
J Bacteriol. 1986 Feb;165(2):564-9. doi: 10.1128/jb.165.2.564-569.1986.
10
Mechanisms of antibiotic resistance in Campylobacter species.弯曲杆菌属细菌的抗生素耐药机制。
Antimicrob Agents Chemother. 1988 Aug;32(8):1107-12. doi: 10.1128/AAC.32.8.1107.
Zotero 插件